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Transcriptional profiles of Sox4 cKO, Sox11 cKO, and Sox4/11 dcKO mouse keratinocytes

ABSTRACT: Transcriptional profiling of Sox4cKO, Sox11cKO, or Sox4/11dcKO mouse keratinocytes as compared to their wild-type control keratinocytes, isolated from gender matched neonatal pairs. Goal was to identify the genes differentially expresssed in the conditional knockout keratinocytes vs the wild-type control.

ORGANISM(S): Mus musculus

PROVIDER: GSE120827 | GEO | 2018/10/05

REPOSITORIES: GEO

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Transcriptional profiles of Sox4 cKO, Sox11 cKO, and Sox4/11 dcKO mouse epidermis at E16

Project description:Transcriptional profiling of mouse E16 epidermis deficient of Sox4, Sox11, or both as compared to gender matched wild type littermate controls. Goal was to identify the genes differentially expresssed in the conditional knockout epidermis vs the wild-type control epidermis.

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Transcriptional profiles of Sox4 cKO, Sox11 cKO, and Sox4/11 dcKO mouse keratinocytes

Project description:Transcriptional profiles of Sox4 cKO, Sox11 cKO, and Sox4/11 dcKO mouse keratinocytes

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Transcriptional profiles of Sox4 cKO, Sox11 cKO, and Sox4/11 dcKO mouse epidermis at E16

Project description:Transcriptional profiles of Sox4 cKO, Sox11 cKO, and Sox4/11 dcKO mouse epidermis at E16

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SOX11 and SOX4 drive the reactivation of an embryonic gene program during wound repair

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Project description:We knocked down SOX4 in T24 cell and created 3 cell lines: T24-scrambled, T24-SOX4-knockdown and T24-SOX4-rescue and compared gene expression changes SOX4 is a developmental transcription factor that is overexpressed in as many as 23% of bladder cancer patients, but the role of SOX4 in bladder cancer tumorigenesis is not well understood. Given SOX4’s many roles in embryonic development and context-dependent regulation of gene expression, we sought to understand SOX4’s contribution to bladder cancer and to elucidate SOX4 regulated genes that might contribute to tumorigenesis. We employed a CRISPR interference (CRISPRi) method to transcriptionally repress SOX4 expression in T24 bladder cancer cell lines, rescued these cell lines with lentivirally expressed SOX4, and performed whole genome expression profiling. SOX4 knockdown cells exhibited decreased invasive capabilities but no changes in migration or proliferation, while rescue with SOX4 lentiviral vector restored the invasive phenotype. Gene expression profiling revealed 173 high confidence SOX4 regulated genes

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Expression data from LNCaP cells transfected with SOX4 cDNA, SOX4 siRNA or GFP cDNA

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