Gene expression analysis of HP1B mutants in D. melanogaster.
Ontology highlight
ABSTRACT:
ORGANISM(S): Drosophila melanogaster
PROVIDER: GSE120841 | GEO | 2018/10/05
REPOSITORIES: GEO
Similar Datasets
Project description:We profile gene expression changes in a mutant strain lacking the D. melanogaster HP1 homolog HP1C at the third instar larval stage. Compared to the yw control strain (previously published) several hundred genes are deregulated.
2021-07-21 | GSE180302 | GEO
Project description:modENCODE_submission_5607 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: HP1b mutant(official name : Hp1b^16 genotype : Hp1b^16/Hp1b^16 outcross : n/a transgene : n/a tags : Transposon tag : GFP description : Allele of HP1b generated by imprecise excision of CB02849. The 539bp deletion removes the Start codon and a large part of the Hp1b coding region. made_by : Nicole Riddle ); Developmental Stage: 3rd Instar Larvae; Genotype: Hp1b^16/Hp1b^16; Transgene: n/a; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain HP1b mutant(official name : Hp1b^16 genotype : Hp1b^16/Hp1b^16 outcross : n/a transgene : n/a tags : Transposon tag : GFP description : Allele of HP1b generated by imprecise excision of CB02849. The 539bp deletion removes the Start codon and a large part of the Hp1b coding region. made_by : Nicole Riddle ); Antibody HP1 wa191 (target is HP1a); Developmental Stage 3rd Instar Larvae
2013-03-06 | E-GEOD-44889 | biostudies-arrayexpress
Project description:HM1, HP1a-/-, and HP1b-/- ESC transcriptomes were generated to determine whether depletion of these HP1 proteins influences gene and/or retroelement expression
2013-05-25 | GSE47370 | GEO
Project description:modENCODE_submission_5604 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: HP1b mutant(official name : Hp1b^16 genotype : Hp1b^16/Hp1b^16 outcross : n/a transgene : n/a tags : Transposon tag : GFP description : Allele of HP1b generated by imprecise excision of CB02849. The 539bp deletion removes the Start codon and a large part of the Hp1b coding region. made_by : Nicole Riddle ); Developmental Stage: 3rd Instar Larvae; Genotype: Hp1b^16/Hp1b^16; Transgene: n/a; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain HP1b mutant(official name : Hp1b^16 genotype : Hp1b^16/Hp1b^16 outcross : n/a transgene : n/a tags : Transposon tag : GFP description : Allele of HP1b generated by imprecise excision of CB02849. The 539bp deletion removes the Start codon and a large part of the Hp1b coding region. made_by : Nicole Riddle ); Antibody HP1b Q4114 (target is HP1b); Developmental Stage 3rd Instar Larvae
2013-03-13 | E-GEOD-45101 | biostudies-arrayexpress
Project description:modENCODE_submission_5573 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: HP1b mutant(official name : Hp1b^16 genotype : Hp1b^16/Hp1b^16 outcross : n/a transgene : n/a tags : Transposon tag : GFP description : Allele of HP1b generated by imprecise excision of CB02849. The 539bp deletion removes the Start codon and a large part of the Hp1b coding region. made_by : Nicole Riddle ); Developmental Stage: 3rd Instar Larvae; Genotype: Hp1b^16/Hp1b^16; Transgene: n/a; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain HP1b mutant(official name : Hp1b^16 genotype : Hp1b^16/Hp1b^16 outcross : n/a transgene : n/a tags : Transposon tag : GFP description : Allele of HP1b generated by imprecise excision of CB02849. The 539bp deletion removes the Start codon and a large part of the Hp1b coding region. made_by : Nicole Riddle ); Antibody HP1b Q4113 (target is HP1b); Developmental Stage 3rd Instar Larvae
2013-03-13 | E-GEOD-45099 | biostudies-arrayexpress
Project description:modENCODE_submission_3017 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: CHIP-chip EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: CME-W1-Cl.8+; Tissue: dorsal mesothoracic disc; Developmental Stage: third instar larval stage; Sex: Male; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line CME-W1-Cl.8+; Antibody HP1b (Henikoff) (target is HP1b)
2011-03-09 | E-GEOD-27744 | biostudies-arrayexpress
Project description:modENCODE_submission_3016 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: ML-DmBG3-c2; Tissue: CNS-derived cell-line; Developmental Stage: third instar larval stage; Genotype: y v f mal; Sex: Unknown; NUMBER OF REPLICATES: 6; EXPERIMENTAL FACTORS: Cell Line ML-DmBG3-c2; Antibody HP1b (Henikoff) (target is HP1b)
2013-02-22 | E-GEOD-44462 | biostudies-arrayexpress
Project description:HM1, HP1a-/-, and HP1b-/- ESC transcriptomes were generated to determine whether depletion of these HP1 proteins influences gene and/or retroelement expression mRNA profiles of HP1a and HP1b Knockouts and its corresponding wildtype
2013-05-25 | E-GEOD-47370 | biostudies-arrayexpress
Project description:modENCODE_submission_5605 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: HP1b mutant(official name : Hp1b^16 genotype : Hp1b^16/Hp1b^16 outcross : n/a transgene : n/a tags : Transposon tag : GFP description : Allele of HP1b generated by imprecise excision of CB02849. The 539bp deletion removes the Start codon and a large part of the Hp1b coding region. made_by : Nicole Riddle ); Developmental Stage: 3rd Instar Larvae; Genotype: Hp1b^16/Hp1b^16; Transgene: n/a; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain HP1b mutant(official name : Hp1b^16 genotype : Hp1b^16/Hp1b^16 outcross : n/a transgene : n/a tags : Transposon tag : GFP description : Allele of HP1b generated by imprecise excision of CB02849. The 539bp deletion removes the Start codon and a large part of the Hp1b coding region. made_by : Nicole Riddle ); Antibody H3K36me3 abcam (target is H3K36me3); Developmental Stage 3rd Instar Larvae
2013-03-06 | E-GEOD-44888 | biostudies-arrayexpress
Project description:modENCODE_submission_5603 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: HP1b mutant(official name : Hp1b^16 genotype : Hp1b^16/Hp1b^16 outcross : n/a transgene : n/a tags : Transposon tag : GFP description : Allele of HP1b generated by imprecise excision of CB02849. The 539bp deletion removes the Start codon and a large part of the Hp1b coding region. made_by : Nicole Riddle ); Developmental Stage: 3rd Instar Larvae; Genotype: Hp1b^16/Hp1b^16; Transgene: n/a; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain HP1b mutant(official name : Hp1b^16 genotype : Hp1b^16/Hp1b^16 outcross : n/a transgene : n/a tags : Transposon tag : GFP description : Allele of HP1b generated by imprecise excision of CB02849. The 539bp deletion removes the Start codon and a large part of the Hp1b coding region. made_by : Nicole Riddle ); Antibody Pc (target is Pc); Developmental Stage 3rd Instar Larvae
2013-03-06 | E-GEOD-44887 | biostudies-arrayexpress