Gene expression analysis of HP1C mutants in D. melanogaster
Ontology highlight
ABSTRACT:
ORGANISM(S): Drosophila melanogaster
PROVIDER: GSE180302 | GEO | 2021/07/21
REPOSITORIES: GEO
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Project description:We profile gene expression changes in two mutant strains lacking the D. melanogaster HP1 homolog HP1B at the third instar larval stage. Compared to the yw control strain, several hundred genes are deregulated, with metabolic genes being over-represented among the deregulated gene set.
2018-10-05 | GSE120841 | GEO
Project description:modENCODE_submission_6075 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: HP1c mutant(official name : HP1c[f04929] genotype : w[1118]; PBac{w[+mC]=WH}HP1c[f04929] outcross : unknown transgene : PBac insertion tags : Transposon description : Insertion of PBac transgene disrupts Hp1c gene (Gene disruption project). Developmental Stage: 3rd Instar Larvae; Genotype: w[1118]; PBac{w[+mC]=WH}HP1c[f04929]; Transgene: PBac insertion; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain HP1c mutant(official name : HP1c[f04929] genotype : w[1118]; PBac{w[+mC]=WH}HP1c[f04929] outcross : unknown transgene : PBac insertion tags : Transposon description : Insertion of PBac transgene disrupts Hp1c gene (Gene disruption project). Antibody HP1 wa191 (target is HP1a); Developmental Stage 3rd Instar Larvae
2013-03-06 | E-GEOD-44897 | biostudies-arrayexpress
Project description:modENCODE_submission_4184 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: HP1c mutant(official name : HP1c[f04929] genotype : w[1118]; PBac{w[+mC]=WH}HP1c[f04929] outcross : unknown transgene : PBac insertion tags : Transposon description : Insertion of PBac transgene disrupts Hp1c gene (Gene disruption project). Developmental Stage: 3rd Instar Larvae; Genotype: w[1118]; PBac{w[+mC]=WH}HP1c[f04929]; Transgene: PBac insertion; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain HP1c mutant(official name : HP1c[f04929] genotype : w[1118]; PBac{w[+mC]=WH}HP1c[f04929] outcross : unknown transgene : PBac insertion tags : Transposon description : Insertion of PBac transgene disrupts Hp1c gene (Gene disruption project). Antibody HP1b Q4114 (target is HP1b); Developmental Stage 3rd Instar Larvae
2013-03-13 | E-GEOD-45058 | biostudies-arrayexpress
Project description:modENCODE_submission_2669 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determe the locations of 125 chromosomal proteins and histone modifications across the Drosophila melanogaster genome. The proteins and modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: CHIP-chip EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: CME-W1-Cl.8+; Tissue: dorsal mesothoracic disc; Developmental Stage: third instar larval stage; Sex: Male; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line CME-W1-Cl.8+; Antibody HP1c (MO 462) (target is HP1c)
2010-08-10 | E-GEOD-23484 | biostudies-arrayexpress
Project description:modENCODE_submission_5112 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using the Illumina NGS platform. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Developmental Stage: 3rd Instar Larvae; Genotype: wild type; EXPERIMENTAL FACTORS: Strain Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Antibody HP1c (MO 462) (target is HP1c); Developmental Stage 3rd Instar Larvae
2013-08-02 | E-GEOD-49495 | biostudies-arrayexpress
Project description:modENCODE_submission_3023 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Developmental Stage: 3rd Instar Larvae; Genotype: wild type; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Antibody HP1c (MO 462) (target is HP1c); Developmental Stage 3rd Instar Larvae
2011-10-12 | E-GEOD-32742 | biostudies-arrayexpress
Project description:modENCODE_submission_3701 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: HP1a mutant Developmental Stage: 3rd Instar Larvae; Genotype: Su(var)205 [4]/Su(var)205 [5]; Transgene: none; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain HP1a mutant Antibody HP1c (MO 462) (target is HP1c); Developmental Stage 3rd Instar Larvae
2013-02-22 | E-GEOD-44475 | biostudies-arrayexpress
Project description:Gene expression analysis of HP1C mutants in D. melanogaster
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2018-01-08 | GSE94915 | GEO
Project description:To investigate how dSETDB1 regulated the genome-wide distribution of HP1 proteins, we performed ChIP-chip assay on the third instar larval lysate from the dSETDB1null mutants in comparison to the wild type.
2014-08-28 | GSE50473 | GEO