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Next Generation Sequencing Facilitates Quantitative Analysis of Control and beta-catenin Deficient Cancer-associated Fibroblasts Transcriptomes

ABSTRACT: D4M BrafV600E; Ptenlox5/lox5 melanoma cells mixed with uninduced control Fb (Ctnnb1+/+; Col1α2-CreER) or mutant bcat/Fb (Ctnnb1fl/fl; Col1α2-CreER) were injected intradermally into flanks of control Ctnnb1+/+; Col1α2-CreER or Ctnnb1 fl/fl; Col1α2-CreER mice, respectively. Tumors were allowed to grow to a volume of approximately 62.5 cubic millimeters when tamoxifen (TAM) was then administrated to induce β-catenin ablation in mutant bcat/Fb CAFs. Control CAFs or bcat/CAFs were collected from BrafV600E; Ptenlox5/lox5 melanoma tumors using PDGFRα as a marker for fibroblasts. Normal fibroblasts were collected from B6 mouse skin.

ORGANISM(S): Mus musculus

PROVIDER: GSE121186 | GEO | 2021/10/11

REPOSITORIES: GEO

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