Genomics

Dataset Information

0

RUNX1-ETO orchestrates dynamic enhancer promoter communication in t(8;21) Acute Myeloid Leukaemia (ChIP-Seq)


ABSTRACT: Acute myeloid leukaemia (AML) is caused by mutations in transcriptional and epigenetic regulator genes impairing myeloid differentiation. The t(8;21)(q22;q22) translocation generates the leukemogenic RUNX1-ETO fusion protein which interferes with the hematopoietic master regulator RUNX1. We previously showed that maintenance of t(8;21) AML is dependent on RUNX1-ETO as its depletion causes extensive changes in transcription factor binding and gene expression as well as myeloid differentiation. How changes in gene expression and binding events are connected within a transcriptional network is unclear. To this end, we assigned cis-regulatory elements to each other using promoter-capture chromosomal conformation assays in the presence and absence of RUNX1-ETO. From these data we constructed a RUNX1-ETO dependent dynamic transcriptional network maintaining AML. Integration of these data with gene expression and transcription factor binding data shows that RUNX1-ETO participates in interactions and that differential cis-element interactions are driven by alterations in the binding of RUNX1-ETO regulated transcription factors.

ORGANISM(S): Homo sapiens

PROVIDER: GSE121280 | GEO | 2019/11/11

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2019-11-11 | GSE121281 | GEO
2014-09-24 | E-GEOD-60130 | biostudies-arrayexpress
2014-09-24 | E-GEOD-54478 | biostudies-arrayexpress
2012-03-26 | E-GEOD-29225 | biostudies-arrayexpress
2010-10-26 | E-GEOD-24384 | biostudies-arrayexpress
2014-09-24 | GSE60130 | GEO
2014-09-24 | GSE54478 | GEO
2010-10-26 | GSE24384 | GEO
2012-03-27 | E-GEOD-29222 | biostudies-arrayexpress
2012-03-27 | E-GEOD-34540 | biostudies-arrayexpress