Genomics

Dataset Information

0

Identification of a dynamic core transcriptional network in t(8;21) AML regulating differentiation block and self-renewal [ChIP-Seq]


ABSTRACT: The leukemogenic fusion protein RUNX1/ETO impairs myeloid differentiation and drives malignant self-renewal. Here we use digital footprinting and ChIP-sequencing to identify the core RUNX1/ETO responsive transcriptional network of t(8;21) cells. We show that the transcriptional programme underlying leukemic propagation is regulated by a dynamic equilibrium between RUNX1/ETO and RUNX1 complexes, which bind in a mutually exclusive fashion to identical genomic sites. Perturbation of this equilibrium by RUNX1/ETO knockdown results in a global reassembly of transcription factor complexes within pre-existing open chromatin and the formation of a new C/EBP-alpha-dominated transcriptional network driving myeloid differentiation. Our work demonstrates that the block in myeloid differentiation in t(8;21) is caused by the dynamic balance between RUNX1/ETO and RUNX1 activities and the repression of C/EBP-alpha and highlights the core targets of epigenetic reprogramming in t(8;21) AML.

ORGANISM(S): Homo sapiens

PROVIDER: GSE60130 | GEO | 2014/09/24

SECONDARY ACCESSION(S): PRJNA257531

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2014-09-24 | E-GEOD-60130 | biostudies-arrayexpress
2014-09-24 | E-GEOD-54478 | biostudies-arrayexpress
2014-09-24 | GSE54478 | GEO
2020-02-17 | PXD017230 | Pride
2012-03-27 | E-GEOD-29222 | biostudies-arrayexpress
2012-03-26 | E-GEOD-29225 | biostudies-arrayexpress
2019-11-11 | GSE121281 | GEO
2019-11-11 | GSE121280 | GEO
2012-03-27 | E-GEOD-34540 | biostudies-arrayexpress
2012-03-27 | E-GEOD-29223 | biostudies-arrayexpress