Genomics

Dataset Information

0

The Protein Phosphatase 4 Complex Promotes Transcription and Processing of Primary microRNAs in Arabidopsis [ncRNA-Seq]


ABSTRACT: Protein phosphatase 4 (PP4) is a highly conserved serine/threonine protein phosphatase in yeast, plants, and animals. The composition and functions of the plant PP4 are poorly understood. Here, we uncovered previously unsuspected complexity of Arabidopsis PP4 and identified the composition of one form of PP4 containing the regulatory subunit PP4R3A. We showed that PP4R3A, together with one of two redundant catalytic subunit genes PPX1 and PPX2, promotes the biogenesis of microRNAs. PP4R3A is a chromatin-associated protein, interacts with RNA polymerase II (Pol II), and recruits Pol II to the promoters of MIR genes to promote their transcription. PP4R3A probably also promotes the cotranscriptional processing of miRNA precursors as it recruits the microprocessor component HYPONASTIC LEAVES1 (HYL1) to MIR genes and nuclear dicing bodies. Moreover, we showed that hundreds of introns exhibit splicing defects in pp4r3a mutants. Together, this study revealed a role of Arabidopsis PP4 in transcription and nuclear RNA metabolism.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE121453 | GEO | 2019/02/08

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2019-02-08 | GSE121450 | GEO
2020-12-02 | GSE154093 | GEO
2020-08-04 | GSE138548 | GEO
2006-08-25 | GSE5597 | GEO
2013-12-16 | E-MTAB-1528 | biostudies-arrayexpress
2019-01-12 | GSE124996 | GEO
2023-05-23 | PXD039878 | Pride
2024-11-26 | GSE271891 | GEO
2024-11-26 | GSE271484 | GEO
2022-05-04 | GSE192782 | GEO