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Genome-wide analysis of RNA Pol II CTD modifications in resonse to lysine deacetylase inhibition

ABSTRACT: To investigate the role of RNA Pol II CTD acetylation we performed comprehensive deacetylase inhibition using common small-molecule inhibitors and performed ChIP-seq from mouse cells using antibodies against RNA Polymerase II CTD modifications: (8WG16, unmodified heptads; K7ac, acetylated heptads; 4H8, S5-phosphorylated heptads). In addition, we tested the effect of knockdown of RPRD1B, a key transcription regulator that recruits RPAP2, a CTD S5-phosphatase.

ORGANISM(S): Mus musculus

PROVIDER: GSE124996 | GEO | 2019/01/12

REPOSITORIES: GEO

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Heptad-specific phosphorylation of RNA polymerase II CTD

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