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Genome-wide assessment of H4K20me1 chromatin profile in D458 shNull and shSETD8 medulloblastoma cells

ABSTRACT: The goal of this study was to examine the H4K20me1 profile in medulloblastoma cells with wild type SETD8 or depleted SETD8.

ORGANISM(S): Homo sapiens

PROVIDER: GSE121556 | GEO | 2019/05/17

REPOSITORIES: GEO

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Genome-wide assessment of H4K20me1 chromatin profile in D458 shNull and shSETD8 medulloblastoma cells

Project description:Genome-wide assessment of H4K20me1 chromatin profile in D458 shNull and shSETD8 medulloblastoma cells

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Transcriptome analysis in shNull and shSETD8 medulloblastoma cells

Project description:The goal of this study was to determine the effect on gene transcription profiles in medulloblastoma cells that were depeleted of the epigenomic modifier SETD8 vs control.

2019-05-17 | GSE121247 | GEO

H4K20me1 plays a dual role in transcriptional regulation during regeneration and axis patterning of Hydra [RNA-seq]

Project description:The evolution of the first body axis in the animal kingdom and an extensive ability to regenerate makes Hydra, a Cnidarian, an excellent model system to understand the underlying epigenetic mechanisms. We identify that SETD8 is critical for regeneration, and H4K20me1 colocalizes with transcriptional activation machinery locally at the β-catenin bound TCF/LEF binding sites on the promoters of head-associated genes, marking an epigenetic activation node. Contrastingly, a global genome-wide analysis of the H4K20me1 occupancy revealed a negative correlation with transcriptional activation. We propose H4K20me1 as a general repressive histone mark in Cnidaria and describe its dichotomous role in transcriptional regulation in Hydra.

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H4K20me1 plays a dual role in transcriptional regulation during regeneration and axis patterning of Hydra [ChIP-seq Reg]

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H4K20me1 plays a dual role in transcriptional regulation during regeneration and axis patterning of Hydra [ChIP-seq ALP]

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The histone methyltransferase Setd8 represses Gata2 expression and regulates erythroid maturation

Project description:The chromatin modifying enzymes that drive the erythroid-specific transcription program are incompletely understood. Setd8 is the sole histone methyltransferase in mammals capable of generating mono-methylated histone H4 lysine 20 (H4K20me1) and is expressed at significantly higher levels in erythroid cells than any other cell- or tissue- type, suggesting that Setd8 has an erythroid-specific function. To test this hypothesis, stable knockdown of Setd8 was established in extensively self-renewing erythroblasts (ESREs), a well-characterized, non-transformed, model of erythroid maturation. Setd8 knockdown impaired erythroid maturation, characterized by a delay in hemoglobin accumulation, larger cell area, persistent kit expression, incomplete nuclear condensation, and lower rates of enucleation than control cells. Setd8 knockdown did not alter ESRE proliferation or viability, or result in accumulation of DNA damage. Global gene expression analyses following Setd8 knockdown suggests that in erythroid cells, Setd8 functions primarily as a repressor and demonstrated high levels of Gata2 expression. Setd8 occupies critical regulatory elements in the Gata2 locus, and knockdown of Setd8 resulted in loss of H4K20me1 and gain of H4 acetylation at the Gata2 1S promoter. Taken together, these results imply that Setd8 is an important regulator of erythroid maturation that works in part through repression of Gata2. RNA-seq was performed of Setd8 knockdown and control cells, both while the cells were proliferating, and after 6 hours of maturation.

2015-04-24 | E-GEOD-62647 | biostudies-arrayexpress

The histone methyltransferase Setd8 represses Gata2 expression and regulates erythroid maturation

2015-04-24 | GSE62647 | GEO

h4k20me1-Establishing a reference epigenome in arabidopsis seedlings

Project description:gfe_epigenome - h4k20me1 - Epigenomic mapping - H4K20me1 ChIP_chip

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Effect of SETD8/PR-Set7 knockdown on gene expression profiles in human fibroblasts

Project description:Cellular senescence is an ireversible growth arrest with alterd metabolic potentials including DNA, RNA and protein dynamics. We found that loss of the SETD8/PR-Set7 methyltransferase, which catalyzes mono-methylation of histone H4 at lysine 20 (H4K20me1), induces senescence in human fibroblasts. To investigate the role of SETD8 in cellular senescence, we performed a microarray-based transcriptomic analysis in SETD8-knockdown cells. Our results demonstrate that SETD8 links the epigenomic gene regulation to senescence-associated metabolic remodeling.

2017-03-30 | GSE86545 | GEO

h4k20me1-Establishing a reference epigenome in arabidopsis seedlings

Project description:gfe_epigenome - h4k20me1 - Epigenomic mapping - H4K20me1 ChIP_chip 2 dye-swap - chip-chip

2011-04-19 | E-GEOD-24654 | biostudies-arrayexpress