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H4K20me1 plays a dual role in transcriptional regulation during regeneration and axis patterning of Hydra [RNA-seq]

ABSTRACT: The evolution of the first body axis in the animal kingdom and an extensive ability to regenerate makes Hydra, a Cnidarian, an excellent model system to understand the underlying epigenetic mechanisms. We identify that SETD8 is critical for regeneration, and H4K20me1 colocalizes with transcriptional activation machinery locally at the β-catenin bound TCF/LEF binding sites on the promoters of head-associated genes, marking an epigenetic activation node. Contrastingly, a global genome-wide analysis of the H4K20me1 occupancy revealed a negative correlation with transcriptional activation. We propose H4K20me1 as a general repressive histone mark in Cnidaria and describe its dichotomous role in transcriptional regulation in Hydra.

ORGANISM(S): Hydra vulgaris

PROVIDER: GSE205916 | GEO | 2023/03/09

REPOSITORIES: GEO

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H4K20me1 plays a dual role in transcriptional regulation during regeneration and axis patterning of Hydra [ChIP-seq ALP]

Project description:The evolution of the first body axis in the animal kingdom and an extensive ability to regenerate makes Hydra, a Cnidarian, an excellent model system to understand the underlying epigenetic mechanisms. We identify that SETD8 is critical for regeneration, and H4K20me1 colocalizes with transcriptional activation machinery locally at the β-catenin bound TCF/LEF binding sites on the promoters of head-associated genes, marking an epigenetic activation node. Contrastingly, a global genome-wide analysis of the H4K20me1 occupancy revealed a negative correlation with transcriptional activation. We propose H4K20me1 as a general repressive histone mark in Cnidaria and describe its dichotomous role in transcriptional regulation in Hydra.

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H4K20me1 plays a dual role in transcriptional regulation during regeneration and axis patterning of Hydra

Project description:H4K20me1 plays a dual role in transcriptional regulation during regeneration and axis patterning of Hydra

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