Transcriptional profile of pmt1 pmt2 mutants
Ontology highlight
ABSTRACT:
ORGANISM(S): Saccharomyces cerevisiae
PROVIDER: GSE12181 | GEO | 2010/09/30
SECONDARY ACCESSION(S): PRJNA114329
REPOSITORIES: GEO
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Project description:We did transcription profiling on the effect of pmt1 pmt4 deletion, genes involved in the O-glycosylation process. These mutants cells show inhibition of mating, filamentation and induction of cell wall compensatory mechanism. Two biological samples were analyzed for each condition, and a microarray experiment were carried out for each sample 1. wild type cells SEY6210 2. pmt1 pmt2 cells from SEY6210 background
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Project description:We did transcription profiling on the effect of O-glycosylation inhibitor OGT2468 in Saccharomyces cerevisiae using strains SEY6210 (wild type). Yeast cells exposed to OGT2468 in YPD growth medium show a significant inhibition of mating, filamentation and induction of cell wall compensatory mechanism. Three biological samples were analyzed for each condition, and a microarray experiment were carried out for each sample 1. absence of the drug (but the corresponding amount of DMSO 1%) 2. presence of 1 microMolar of OGT2468
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