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Genome-wide identification of protein binding sites on RNAs in mammalian cells [PBS-R]

ABSTRACT: We develop a novel method, called protein binding sites on RNA (PBS-R) to identify genome-wide protein binding sites on RNAs without using antibodies. Double click strategy is used for the PBS-R assay. Proteins and RNAs are UV-crosslinked in vivo first, then the proteins are crosslinked to the magnetic beads. The protein binding RNA elements are captured, reverse transcribed and sequenced.

ORGANISM(S): Homo sapiens

PROVIDER: GSE121919 | GEO | 2018/11/10

REPOSITORIES: GEO

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Genome-wide identification of protein binding sites on RNAs in mammalian cells [PBS-R]

Project description:Genome-wide identification of protein binding sites on RNAs in mammalian cells [PBS-R]

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RNA-Seq of A375 cells treated with tetracycline-based crosslinking probes, with and without competition by parent tetracyclines, to show specific binding of the tetracycline analogs Col-3 and doxycycline on rRNA substructures of the human ribosome

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