Proteomics

Dataset Information

0

Saccharomyces cerevisiae mRBPome


ABSTRACT: This project aims to identify novel RNA binding proteins in the baker's yeast, Saccharomyces cerevisiae. Since interactions between RNAs and proteins may be transient, yeast cells were crosslinked with UV light at 254 nm which promotes the covalent link between proteins and RNAs. After this, polyadenylated mRNAs were purified via oligo(dT) coupled to magentic beads under stringet conditions. Finally, samples were subjected to mass spectrometry analysis. To rule out the possibility of RNA-independent binding we also analysed other samples: i) samples digested with RNase one; ii) samples where we performed competition assays with polyadenylic acid.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Ana Matia Gonzalez  

LAB HEAD: ANDRE P. GERBER

PROVIDER: PXD002293 | Pride | 2015-10-26

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
WT_Yeast_1.raw Raw
WT_Yeast_2.raw Raw
WT_Yeast_3.raw Raw
WT_Yeast_Competition.msf Msf
WT_Yeast_RNaseOne_2.msf Msf
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Publications

Conserved mRNA-binding proteomes in eukaryotic organisms.

Matia-González Ana M AM   Laing Emma E EE   Gerber André P AP  

Nature structural & molecular biology 20151123 12


RNA-binding proteins (RBPs) are essential for post-transcriptional regulation of gene expression. Recent high-throughput screens have dramatically increased the number of experimentally identified RBPs; however, comprehensive identification of RBPs within living organisms is elusive. Here we describe the repertoire of 765 and 594 proteins that reproducibly interact with polyadenylated mRNAs in Saccharomyces cerevisiae and Caenorhabditis elegans, respectively. Furthermore, we report the different  ...[more]

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