Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chromosome conformation capture by high thoughput sequencing (HiC) during Naïve CD4 T cell activation

ABSTRACT: We report chromatin interaction changes before and early after naïve CD4 T cell activation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE122051 | GEO | 2021/10/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Chromosome conformation capture by high thoughput sequencing (HiC) during Naïve CD4 T cell activation

Project description:Chromosome conformation capture by high thoughput sequencing (HiC) during Naïve CD4 T cell activation

| PRJNA503263 | ENA

Human Fetal and Adult Peripheral Naïve CD4+ T cells and CD4+CD25+ Treg cells

Project description:We compared differences in fetal and adult T cells by performing whole genome profiling on sort-purified T cells (naïve CD4+ and Treg cells) from human fetal specimens (18-22 gestational weeks) and adult specimens (age 25-40 years old). Fetal and Adult Naïve CD4+ T cells phenotype: CD3+CD4+CD45RA+CCR7+CD27+, Fetal and Adult CD4+CD25+ Treg phenotype: CD3+CD4+CD25bright Four different groups were analyzed: Fetal Naïve CD4+ T cells, Adult Naïve CD4+ T cells, Fetal Treg cells, Adult Treg cells. For each group three independent donors were analyzed.

2010-12-17 | E-GEOD-25087 | biostudies-arrayexpress

H3K27me3 and JMJD3 ChIP-seq during naïve CD4 T cell activation

Project description:Histone modifications have been shown critically involved in transcriptional regulation, but the functions of histone modifiers and the impact of histone modifications in early transcription induction post CD4 T cell activation are unclear. We show here that JMJD3 binds to chromatin rapidly after naïve CD4 T cell activation, which is correlated with removal of H3K27me3 at promoter, enhancer and intronic regions.

2021-10-01 | GSE122219 | GEO

Bcl6 controlled gene expression in naïve CD4 T cells (Tf0)

Project description:Gene expression data from wild-type and Bcl6-/- naive CD4 T cells In order to find genes regulated by Bcl6 in follicular helper T cells Naïve CD4 T cells were sorted from wild-type (WT) and T cell-specific conditional Bcl6-/- (KO) mice-- 8 samples, 4 WT and 4 KO

2015-04-08 | E-GEOD-67622 | biostudies-arrayexpress

Bcl6 controlled gene expression in naïve CD4 T cells (Tfh)

2015-04-08 | E-GEOD-67623 | biostudies-arrayexpress

Capture-C: a modular and flexible approach for high-resolution chromosome conformation capture.

Project description:Chromosome conformation capture (3C) methods measure the spatial proximity between DNA elements in the cell nucleus. Many methods have been developed to sample 3C material, including the Capture-C family of protocols. Capture-C methods use oligonucleotides to enrich for interactions of interest from sequencing-ready 3C libraries. This approach is modular and has been adapted and optimized to work for sampling of disperse DNA elements (NuTi Capture-C), including from low cell inputs (LI Capture-C), as well as to generate Hi-C like maps for specific regions of interest (Tiled-C) and to interrogate multiway interactions (Tri-C). We present the design, experimental protocol and analysis pipeline for NuTi Capture-C in addition to the variations for generation of LI Capture-C, Tiled-C and Tri-C data. The entire procedure can be performed in 3 weeks and requires standard molecular biology skills and equipment, access to a next-generation sequencing platform, and basic bioinformatic skills. Implemented with other sequencing technologies, these methods can be used to identify regulatory interactions and to compare the structural organization of the genome in different cell types and genetic models.

| S-EPMC7613269 | biostudies-literature

Human Fetal and Adult Peripheral Naïve CD4+ T cells and CD4+CD25+ Treg cells

2010-12-17 | GSE25087 | GEO

Comparison of gene expression in mouse CD4 naïve and activated T-cells

Project description:Gene expression changes when naïve T-cells are stimulated with antigen. We have stimulated these cells with surrogate antigens (aCD3-aCD28 coated beads) for 48 hours, and then did microarray experiment to identify differentially exprssed genes in the two conditions CD4 naïve T-cells from C57/Bl6 mouse were stimulated (activated) with aCD3-aCD28 coated beads for 48 hours and used for microarray experiment

2011-12-01 | E-GEOD-30196 | biostudies-arrayexpress

Stable Chromosome Condensation Revealed by Chromosome Conformation Capture.

Project description:Chemical cross-linking and DNA sequencing have revealed regions of intra-chromosomal interaction, referred to as topologically associating domains (TADs), interspersed with regions of little or no interaction, in interphase nuclei. We find that TADs and the regions between them correspond with the bands and interbands of polytene chromosomes of Drosophila. We further establish the conservation of TADs between polytene and diploid cells of Drosophila. From direct measurements on light micrographs of polytene chromosomes, we then deduce the states of chromatin folding in the diploid cell nucleus. Two states of folding, fully extended fibers containing regulatory regions and promoters, and fibers condensed up to 10-fold containing coding regions of active genes, constitute the euchromatin of the nuclear interior. Chromatin fibers condensed up to 30-fold, containing coding regions of inactive genes, represent the heterochromatin of the nuclear periphery. A convergence of molecular analysis with direct observation thus reveals the architecture of interphase chromosomes.

| S-EPMC4639323 | biostudies-literature

H3K27me3 and JMJD3 ChIP-seq during naïve CD4 T cell activation

Project description:H3K27me3 and JMJD3 ChIP-seq during naïve CD4 T cell activation

| PRJNA504007 | ENA