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Integrated transcriptional regulatory network of quorum sensing, replication, and SOS response in Dinoroseobacter shibae

ABSTRACT: Transcriptional response of the QS-negative D. shibae strain ∆luxI1 towards externally added 500nM autoinducer (AI) 3-oxo-C14-HSL over a time period of 3 hours.

ORGANISM(S): Dinoroseobacter shibae

PROVIDER: GSE122111 | GEO | 2018/11/03

REPOSITORIES: GEO

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Response to different N-acyl homoserine lactones and N-acyl homoserine lactones-pretreated plants to secondary challenge with flg22.

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ALI1 interacts with N-acyl homoserine lactone and is involved in the perception of the quorum sensing molecule

Project description:In this report, we investigated oxo-C14-HSL-interacting proteins in plants. We propose the AHL-Interacting Protein 1 (ALI1) (previously known as AtGalk2) as interacting protein for oxo-C14-HSL in Arabidopsis. We present here several levels of confirmation and suggest that ALI1 is required for the perception of oxo-C14-HSL in Arabidopsis. This is the first direct evidence of a plant protein interacting with a bacterial quorum sensing molecule.

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Response to different N-acyl homoserine lactones and N-acyl homoserine lactones-pretreated plants to secondary challenge with flg22.

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Project description:Dinoroseobacter shibae overview

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Project description:Comparison of a quorum sensing null mutant supplemented with C18-HSL, C18en-HSL, C18dien-HSL with D. shibae wild-type in order to identify traits induced by distinct autoinducers in this organism. Loop-design, two to three biological replicates of D. shibae wild-type, delta-luxI1 and delta-luxI1 supplemented with 500 nM C18-HSL, C18en-HSL and C18dien-HSL at cultivation start. Samples were taken in mid-exponential growth phase at OD600 0.4

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Comparative analysis of D. shibae wild-type vs. delta-luxI1 supplemented with different acetylated homoserine-lactones

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