Transcriptomics

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Identification of transformation-related pathways in a breast epithelial cell model using a ribonomics approach.


ABSTRACT: Employing MCT-1 oncogene mediated transformation of immortalized breast epithelial MCF10A cells; we characterized the largely reciprocal association of these two RBPs with target mRNAs and their influence on protein expression vis-à-vis cellular transformation. Using a ribonomics approach, we identified mRNAs from cancer-related pathways whose association with AUF1 and/or HuR were altered when comparing immortalized with transformed MCF10A cells. Significantly, we were able to demonstrate that knockdown of HuR expression using RNA interference, reduced anchorage-independent growth capacity in transformed MCF10A cells as well as decreased protein expression of a number of validated target genes. Our data demonstrate that the global alterations in binding of HuR and AUF1 with target transcripts have a critical role in post-transcriptional regulation of genes encoding proteins involved in breast epithelial cell transformation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE12215 | GEO | 2008/07/29

SECONDARY ACCESSION(S): PRJNA113731

REPOSITORIES: GEO

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