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High-throughput single-cell transcriptome profiling of plant cell types

ABSTRACT: Single-cell transcriptome analysis of heterogeneous tissues can provide high-resolution windows into the spatiotemporal dynamics of developmental processes and environmental responses. Here we use the high-hroughput Drop-seq approach to profile the transcriptomes of over 12,000 individual cells from the Arabidopsis root. We identify marker genes for a diversity of cell types, capture cell-type frequency and gene expression alterations in response to sucrose, and illuminate the transcriptome changes that occur across endodermis development.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE122687 | GEO | 2019/05/13

REPOSITORIES: GEO

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High-throughput single cell transcriptome profiling of plant tissue

Project description:Plant tissues are composed of diverse cell populations, but their individual transcriptional profiles are not well characterized. High-throughput single cell RNA sequencing technologies can deconvolute heterogeneous tissues but have not been adopted for plants. Exploiting Arabidopsis root protoplasts as a model, we used Drop-seq to profile >4,000 individual plant cells, capturing a diversity of cell types, identifying novel marker genes, and illuminating developmental trajectories of endodermis and hair cells.

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| S-EPMC4876123 | biostudies-literature

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