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Fermentation product with new equol-producing Lactobacillus paracasei as a probiotic candidate for prevention of skin and intestinal disorder (RNA-Seq)

ABSTRACT: Equol is one of major isoflavones with an affinity to endoplasmic reticulum and has an estrogen-like biological activity. Equol-producing bacteria have been isolated and characterized, however fermentation has been performed in an anaerobic condition with soybean-based products as substrates. Pueraria lobata has been reported as a plant with a higher content of isoflavones, such as daidzein, daidzin, and puerarin.

ORGANISM(S): Lacticaseibacillus paracasei

PROVIDER: GSE122878 | GEO | 2020/10/02

REPOSITORIES: GEO

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Fermentation product with new equol-producing Lactobacillus paracasei as a probiotic candidate for prevention of skin and intestinal disorder (RNA-Seq)

Project description:Fermentation product with new equol-producing Lactobacillus paracasei as a probiotic candidate for prevention of skin and intestinal disorder (RNA-Seq)

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Probiotic Properties of Exopolysaccharide-Producing Lactobacillus Strains Isolated from Tempoyak.

Project description:Tempoyak is a functional Malaysian food (an acid-fermented condiment) which is produced from the pulp of the durian (Durio zibethinus) fruit. The current study aimed to isolate and identify potential exopolysaccharide (EPS)-producing Lactobacillus strains from tempoyak for potential use as probiotics. Seven isolates (DUR2, DUR4, DUR5, DUR8, DUR12, DUR18, and DUR20) out of 44 were able to produce EPS, and exhibited resistance to acid and bile salt compared to the reference strains Lactobacillus rhmnosus (ATCC53103) and L. plantarum (ATCC8014). The seven isolated strains belonged to five different species-L. plantarum, L. fermentum, L. crispatus, L. reuteri, and L. pentosus-which were identified using API 50 CHL and 16S rRNA gene sequences (Polymerase chain reaction, PCR - based). The seven strains displayed different ability to produce EPS (100-850 mg/L). Isolates exhibited a high survivability to acid (pH 3.0), bile salts (0.3%), and gastrointestinal tract model (<70%). Results showed that the auto-aggregation and cell surface hydrophobicity ranged from 39.98% to 60.09% and 50.80% to 80.53%, respectively, whereas, the highest co-aggregation value (66.44%) was observed by L. fermentum (DUR8) with Pseudomonas aeruginosa. The isolates showed good inhibitory activity against tested pathogens, high antioxidant activity (32.29% to 73.36%), and good ability to reduce cholesterol (22.55% to 75.15%). Thus, the seven tested strains have value as probiotics.

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Transcriptome analysis of probiotic Lactobacillus casei Zhang during fermentation in soymilk

2011-12-31 | E-GEOD-23652 | biostudies-arrayexpress

A comparative analysis of genistein and daidzein in affecting lipid metabolism in rat liver

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A comparative analysis of genistein and daidzein in affecting lipid metabolism in rat liver

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Gene expression profile of probiotic Lactobacillus casei Zhang during the late stage of milk fermentation

Project description:Lactobacillus casei Zhang is a probiotic bacterium isolated from koumiss in Inner Mongolia of China that has been commercially used as a starter in the manufacture of dairy products. To study the gene expression profiles of L. casei Zhang during growth in milk, a whole-genome microarray was used. Compared to L. casei Zhang grown to late logarithmic phase in milk, 61 genes were significantly up-regulated (>5 fold) in stationary phase, whereas 26 genes were down-regulated. Collectively, these data showed that the majority of the identified genes was involved in carbohydrate metabolism and energy production, followed by genes involved in nucleotide metabolism, inorganic ion transport, amino acid transport and metabolism, chaperone, etc. This study demonstrates the fundamental effects of cultural conditions on the transcriptome of L. casei Zhang. Moreover, it improves the understanding of the growth and survival mechanism of the bacterium during the late stage of milk fermentation.

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Effect of fermentation with <i>Lactobacillus fermentum</i> FL-0616 on probiotic-rich bean powders.

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Optimization of Fermentation Conditions and Product Identification of a Saponin-Producing Endophytic Fungus.

Project description:BackgroundSome fungal endophytes isolated from P. ginseng may present a new method of obtaining saponins. This experiment aimed to optimize the total saponin yield produced through in vitro fermentation by an endophytic fungus and analyze its saponin species in the fermented extract.MethodsFermentation protocols were optimized with a uniform design and verified through regression analysis to maximize the total saponin yield. The saponin types under optimal fermentation conditions were then identified and analyzed using Liquid Chromatography-Mass Spectrometry.ResultsThe Trametes versicolor strain NSJ105 (gene accession number: OR144428) isolated from wild ginseng could produce total saponins. The total saponin yield could be increased more than two-fold through the optimization of fermentation conditions. The concentration of the total saponins achieved by the verified protocol 105-DP was close to the predicted value. The fermentation conditions of the 105-DP protocol were as follows: potato concentration 97.3 mg/mL, glucose concentration 20.6 mg/mL, inoculum volume 2.1%, fermentation broth pH 2.1, fermentation temperature 29.2 °C, and fermentation time 6 d. It was detected and analyzed that the fermented extract of 105-DP contained the ginsenosides Rf and Rb3.ConclusionThe endophytic fungus Trametes versicolor strain NSJ105 has potential application value in saponin production.

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