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Single-cell and spatial transcriptomics reveal somitogenesis in gastruloids

ABSTRACT: Gastruloids are three-dimensional aggregates of embryonic stem cells (ESCs) that display key features of mammalian post-implantation development, including germ layer specification and axial organization. Gastruloids have mostly been characterized with microscopy-based approaches, limiting the number of genes that can be explored. It is therefore unclear to what extent gene expression in gastruloids reflects in vivo embryonic expression. Using both single-cell RNA-seq (scRNA-seq) and spatial transcriptomics we systematically compared cell types and spatial expression patterns between mouse gastruloids and mouse embryos.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE123187 | GEO | 2019/12/05

REPOSITORIES: GEO

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