Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

GLUD1 blockade triggers glutamine production by macrophages, nourishing satellite cells and promoting muscle regeneration

ABSTRACT: RNAseq was performed on Glud1-deficient macrophages vs. WT macrophages sorted at baseline and at the peak of muscle damage (1day after intramuscular injection of cardiotoxin). By analysing the clustered for M1 and M2 genes as well as for wound healing genes of Glud1-deficient macrophages vs. WT macrophages recovered from muscle damage model, our understanding of key macrophage-dependent events in the damaged muscles will be improved.

ORGANISM(S): Mus musculus

PROVIDER: GSE123825 | GEO | 2020/07/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

GeneChip expression profiling of Glud1 (glutamate dehydrogenase 1) transgenic mice

Project description:Glud1 (glutamate dehydrogenase 1) transgenic mice release more excitatory neurotransmitter glutamate to synaptic cleft throughout lifespan and show signs of accelerated aging. Here we compared transcriptomic profiles of these animals to their wild-type counterparts. The hippocampus was used for the analysis. Keywords: transgenic analysis Three Glud1 transgenic mice vs. three age-matched wide-type mice. Age: 9-month-old. Tissue: hippocampus.

2010-07-12 | E-GEOD-11419 | biostudies-arrayexpress

GLUD1 determines murine muscle stem cell fate by controlling mitochondrial glutamate levels

Project description:Muscle stem cells (MuSCs) enable muscle growth and regeneration after exercise or injury, but how metabolism controls their regenerative potential is poorly understood. We describe that primary metabolic changes can determine murine MuSC fate decisions. We found that glutamine anaplerosis into the TCA cycle decreases during MuSC differentiation and coincides with decreased expression of the mitochondrial glutamate deaminase GLUD1. Deletion of Glud1 in proliferating MuSCs resulted in precocious differentiation and fusion combined with loss of self-renewal in vitro and in vivo. Mechanistically, deleting Glud1 caused mitochondrial glutamate accumulation and inhibited the malate-aspartate shuttle (MAS). The resulting defect in transporting NADH reducing equivalents into the mitochondria induced compartment-specific NAD+/NADH ratio shifts. MAS activity restoration or directly altering NAD+/NADH ratios normalised myogenesis. In conclusion, GLUD1 prevents deleterious mitochondrial glutamate accumulation and inactivation of the MAS in proliferating MuSCs. It thereby acts as a compartment specific metabolic brake on MuSC differentiation.

2024-07-02 | GSE243640 | GEO

GeneChip expression profiling of Glud1 (glutamate dehydrogenase 1) transgenic mice across age

Project description:Glud1 (Glutamate dehydrogenase 1) transgenic mice release more excitatory neurotransmitter glutamate to synaptic cleft throughout lifespan. Here we compared transcriptomic profiles of these animals to their wild-type counterparts across 5 ages. The hippocampus was used for the analysis. Longitudinal studies of Glud1 transgenic and wide-type mice across 5 age points: 10 days post birth, 4.5 mo, 9 mo, 14.5 mo, and 20 mo.

2014-07-31 | E-GEOD-48911 | biostudies-arrayexpress

GLUD1 blockade triggers glutamine production by macrophages, nourishing satellite cells and promoting muscle regeneration

Project description:GLUD1 blockade triggers glutamine production by macrophages, nourishing satellite cells and promoting muscle regeneration

| PRJNA509926 | ENA

GeneChip expression profiling of Glud1 (glutamate dehydrogenase 1) transgenic mice

2010-07-12 | GSE11419 | GEO

GeneChip expression profiling of Glud1 (glutamate dehydrogenase 1) transgenic mice across age

2014-07-31 | GSE48911 | GEO

Chromatin accessibility maps of muscle infiltrating macrophages.

Project description:Genome-wide chromatin accessibility maps of muscle infiltrating macrophages isolated at day 1 to 4 following acute sterile cardiotoxin injury.

2019-07-02 | GSE129393 | GEO

Tissue macrophage subsets derived from regenerating muscle

Project description:Muscle injury was elicited by cardiotoxin injection into the tibialis anterior muscle. Macrophages were isolated 2 days post-injury from the regenerating muscle. We used microarray to obtain global gene expression data of muscle-derived tissue macrophage subsets.

2014-01-02 | GSE44057 | GEO

Identification of a macrophage PPARγ-GDF3 regulatory axis controlling muscle regeneration

2016-10-24 | GSE71155 | GEO

Expression data of CD11b+ cells isolated from WT and C3aR knockout mice after CTX injury

Project description:Regeneration of skeletal muscle following injury is accompanied by transient inflammation initiation and resolution. However, it is unclear what signals control these processes. To better understand the biological pathways by C3a-C3aR activation in monocyte druing muscle regeneration,we examined global transcriptional changes in macrophages from the muscle after CTX injury.Male C57BL/6J mice were used at 12 weeks of age. 30 ul 10uM Cardiotoxin (CTX) was injected to TA muscle to injury muscle. CD11b+ cells was isolated from WT and C3aR-/- muscle at 1 day after CTX injury by FACS , then total RNA obtained from these cells were used for the analysis of RNA-seq.

2017-08-31 | E-MTAB-5886 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data