Transcriptomics

Dataset Information

0

The pause-initiation limit restricts transcription activation in human cells


ABSTRACT: Eukaryotic gene transcription is often controlled at the level of RNA polymerase II (Pol II) pausing in the promoter-proximal region. Pausing Pol II limits the frequency of transcription initiation (‘pause-initiation limit’), predicting that the pause duration must be decreased for transcriptional activation. To test this prediction, we conducted a genome-wide kinetic analysis of the heat shock response in human cells. We show that the pause-initiation limit restricts transcriptional activation at most genes. Gene activation generally requires the activity of the P-TEFb kinase CDK9, which decreases the duration of Pol II pausing and thereby enables an increase in the productive initiation frequency. The transcription of enhancer elements is generally not pause-limited and can be activated without CDK9 activity. Our results define the kinetics of Pol II transcriptional regulation in human cells at all gene classes during a natural transcription response.

ORGANISM(S): Homo sapiens

PROVIDER: GSE123980 | GEO | 2019/07/10

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2017-10-09 | GSE96056 | GEO
2020-05-28 | GSE132764 | GEO
2017-04-17 | GSE85741 | GEO
2017-04-17 | GSE85740 | GEO
2017-04-17 | GSE85739 | GEO
2017-04-17 | GSE85738 | GEO
2022-08-12 | PXD022962 | Pride
2021-02-03 | GSE138358 | GEO
2021-02-03 | GSE138357 | GEO
2021-02-03 | GSE138356 | GEO