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GRO-seq on MCF7 cells induced with sgRNA vectors targeting CTCF binding elements

ABSTRACT: We performed a CRISPR-based functional genetic screen targeting CTCF binding elements (CBEs) located in the vicinity of ERα-bound enhancers. The screen identified four functional CBEs whose targeting resulted in a marked negative effect on the proliferation of MCF-7 cells (ERα-positive cells) but no significant effect on MDA-MB-231 (ERα-negative cells) cells. Here, we carried out GRO-seq analysis on MCF-7 cells induced with an sgRNA vector targeting one of the hits detected by the screen: sgRNA_1118, as well as non-targeting sgRNA and sg1830 as control.

ORGANISM(S): Homo sapiens

PROVIDER: GSE124572 | GEO | 2019/11/01

REPOSITORIES: GEO

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