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GRO-seq on MCF7 cells induced with sgRNA vectors targeting CTCF binding elements

ABSTRACT: GRO-seq on MCF7 cells induced with sgRNA vectors targeting CTCF binding elements

PROVIDER: PRJNA512524 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8384736.fastq.gz	Fastqsanger.gz
	SRR8384737.fastq.gz	Fastqsanger.gz
	SRR8384738.fastq.gz	Fastqsanger.gz

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GRO-seq on MCF7 cells induced with sgRNA vectors targeting CTCF binding elements

Project description:We performed a CRISPR-based functional genetic screen targeting CTCF binding elements (CBEs) located in the vicinity of ERα-bound enhancers. The screen identified four functional CBEs whose targeting resulted in a marked negative effect on the proliferation of MCF-7 cells (ERα-positive cells) but no significant effect on MDA-MB-231 (ERα-negative cells) cells. Here, we carried out GRO-seq analysis on MCF-7 cells induced with an sgRNA vector targeting one of the hits detected by the screen: sgRNA_1118, as well as non-targeting sgRNA and sg1830 as control.

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RNA-seq on MCF7 cells induced with sgRNA vectors targeting CTCF binding elements

Project description:We performed a CRISPR-based functional genetic screen targeting CTCF binding elements (CBEs) located in the vicinity of ERα-bound enhancers. The screen identified four functional CBEs whose targeting resulted in a marked negative effect on the proliferation of MCF-7 cells (ERα-positive cells) but no significant effect on MDA-MB-231 (ERα-negative cells) cells. We then carried out RNA-seq analysis on MCF-7 cells induced with sgRNA vectors targeting these four CBEs: sgRNA_1118, sgRNA_1659, sgRNA_680 and sgRNA_810, as well as non-targeting sgRNA as control.

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RNA-Seq and GRO-seq on MCF7 cells induced with sgRNA vectors targeting CTCF binding elements

Project description:This SuperSeries is composed of the SubSeries listed below.

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