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Detecting cytosine-5 methylation in RNA

ABSTRACT: We generated a map of NSUN6-dependent RNA 5 methyl-cytosine in the human transcriptome by applying RNA bisulfite conversion and sequencing to two sets of cell lines (HUES9 and HEK293) where the RNA cytosine 5 methyl-transfearse NSUN6 has been either knocked out by CRISPR Cas9 or overexpressed using a PiggyBac vector. We apply this and other molecular methods to validate NSUN6 RNA methylation targets with single nucelotide resolution in the human transcriptome.

ORGANISM(S): Homo sapiens

PROVIDER: GSE125046 | GEO | 2019/03/22

REPOSITORIES: GEO

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