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Pioneer and nonpioneer factor cooperation drives lineage specific chromatin opening

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE125671 | GEO | 2019/07/29

REPOSITORIES: GEO

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Pioneer and nonpioneer factor cooperation drives lineage specific chromatin opening.

Project description:Pioneer transcription factors are characterized by having the unique property of enabling the opening of closed chromatin sites, for implementation of cell fates. We previously found that the pioneer Pax7 specifies melanotrope cells through deployment of an enhancer repertoire, which allows binding of Tpit, a nonpioneer factor that determines the related lineages of melanotropes and corticotropes. Here, we investigate the relation between these two factors in the pioneer mechanism. Cell-specific gene expression and chromatin landscapes are defined by scRNAseq and chromatin accessibility profiling. We find that in vivo deployment of the melanotrope enhancer repertoire and chromatin opening requires both Pax7 and Tpit. In cells, binding of heterochromatin targets by Pax7 is independent of Tpit but Pax7-dependent chromatin opening requires Tpit. The present work shows that pioneer core properties are limited to the ability to recognize heterochromatin targets and facilitate nonpioneer binding. Chromatin opening per se may be provided through cooperation with nonpioneer factors.

| S-EPMC6707328 | biostudies-literature

Pioneer and nonpioneer factor cooperation drives lineage specific chromatin opening [scRNA-seq]

Project description:Pioneer transcription factors are coined as having the unique property of “opening closed chromatin sites” for implementation of cell fates. We previously showed that the pioneer Pax7 specifies melanotrope cells through deployment of an enhancer repertoire: this allows binding of Tpit, a nonpioneer factor that determines the related lineages of melanotropes and corticotropes. Here, we investigated the relation between these two factors in the pioneer mechanism. Cell-specific gene expression and chromatin landscapes were defined by scRNAseq and chromatin accessibility profiling. We found that in vivo deployment of the melanotrope enhancer repertoire and chromatin opening requires both Pax7 and Tpit. In cells, binding of heterochromatin targets by Pax7 is independent of Tpit but Pax7-dependent chromatin opening requires Tpit. The present work shows that pioneer core properties are limited to the ability to recognize heterochromatin targets and facilitate nonpioneer binding. Chromatin opening per se may be provided through cooperation with nonpioneer factors.

2019-07-29 | GSE125670 | GEO

Pioneer and nonpioneer factor cooperation drives lineage specific chromatin opening [ChIP-seq]

2019-07-29 | GSE125669 | GEO

Pioneer and nonpioneer factor cooperation drives lineage specific chromatin opening [ATAC-seq]

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Pioneer and nonpioneer factor cooperation drives lineage specific chromatin opening

Project description:Pioneer and nonpioneer factor cooperation drives lineage specific chromatin opening

| PRJNA517135 | ENA

Pioneer and nonpioneer factor cooperation drives lineage specific chromatin opening [scRNA-seq]

Project description:Pioneer and nonpioneer factor cooperation drives lineage specific chromatin opening [scRNA-seq]

| PRJNA517137 | ENA

Pioneer and nonpioneer factor cooperation drives lineage specific chromatin opening [ATAC-seq]

Project description:Pioneer and nonpioneer factor cooperation drives lineage specific chromatin opening [ATAC-seq]

| PRJNA517139 | ENA

Pioneer and nonpioneer factor cooperation drives lineage specific chromatin opening [ChIP-seq]

Project description:Pioneer and nonpioneer factor cooperation drives lineage specific chromatin opening [ChIP-seq]

| PRJNA517138 | ENA

Pioneer factor Pax7 initiates two-step cell-cycle dependent chromatin opening

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-10-08 | GSE240090 | GEO

Chromatin opening ability of pioneer factor Pax7 depends on unique isoform and C-terminal domain

Project description:This SuperSeries is composed of the SubSeries listed below.

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