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Transcriptional responses to glutaminyl tRNA synthetase Gln4p depletion in Saccharomyces cerevisiae

ABSTRACT: A tet-off strain of Saccharomyces cerevisiae was constructed in which the GLN4 glutamine tRNA synthetase gene was placed under control of a doxycycline-regulated promoter. The transcriptional responses to Gln4p tRNA synthetase depletion were assessed by growth of the strain in the presence, or absence, of doxycycline (1 µg/ml). A control, wild-type strain was similarly treated with doxycycline or left untreated as a reference. Each strain/condition RNA isolation was performed using triplicate independent biological samples A, B and C.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE126435 | GEO | 2019/04/01

REPOSITORIES: GEO

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