Transcriptomics

Dataset Information

0

Transcriptional profiling (RNA-seq) of wild-type and Arhgap35 knockout embryonic motor neurons (E12.5)


ABSTRACT: Ventral spinal cord regions micro-dissected from E12.5 Arhgap35 (aka, p190) knockout embryos or control littermates (wild-type or p190+/-) were pulled and dissociated with papain (Worthington Biochemical Cat# LK003153) according to the manufacturer’s instructions. Cells were sorted on a Becton Dickinson FACS Vantage SE DiVa using Coherent Sapphire 488 nm solid state laser and collected directly into RLT lysis buffer containing β-mercaptoethanol (Qiagen). RNA was isolated using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion (Qiagen RNase-Free DNase Set). Each sample contained between 100,000 and 200,000 GFP+ motor neurons isolated from 2-5 spinal cords during a single experimental session. Five p190-/- and five control RNA samples were quantified with Agilent 2200 TapeStation system prior to preparation of mRNA-sequencing libraries (50 bp single-end) using the Illumina TruSeq RNA Library Preparation Kit (v2) according to the manufacturer’s instructions. Libraries were sequenced with Illumina HiSeq 2500 platform. "Negative" samples are from FACS-sorted Hb9:GFP-negative cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE126534 | GEO | 2019/05/01

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2022-01-07 | GSE181501 | GEO
2018-03-23 | GSE107284 | GEO
2023-06-30 | GSE173938 | GEO
2016-07-12 | GSE74690 | GEO
2023-06-27 | E-MTAB-12345 | biostudies-arrayexpress
2016-07-11 | GSE71139 | GEO
2024-04-01 | GSE171561 | GEO
2016-02-05 | GSE77560 | GEO
2020-06-07 | GSE134510 | GEO
2024-06-04 | GSE255947 | GEO