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RNA-sequencing of the adult female fly guts (w1118) with and without oral infection with Pseudomonas entomophila (Pe) or Erwinia carotovora carotovora 15 (Ecc15)

ABSTRACT: Adult female Drosophila melanogaster flies from the w1118 background were fed sucrose, Pseudomonas entomophila (Pe), or Erwinia carotovora carotovora 15 (Ecc15).

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE128489 | GEO | 2019/10/07

REPOSITORIES: GEO

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RNA-sequencing of the adult female fly guts (w1118) with and without oral infection with Pseudomonas entomophila (Pe) or Erwinia carotovora carotovora 15 (Ecc15)

Project description:RNA-sequencing of the adult female fly guts (w1118) with and without oral infection with Pseudomonas entomophila (Pe) or Erwinia carotovora carotovora 15 (Ecc15)

| PRJNA527837 | ENA

RNA-sequencing of the female adult fly guts with enterocyte-specific overexpression and silencing of lark in control and Pseudomonas entomophila infected states.

Project description:The lark gene was either overexpressed or silenced in adult enterocytes. Flies were fed sucrose or Pseudomonas entomophila (Pe).

2019-10-07 | GSE128488 | GEO

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Project description:we investigated changes in transcriptome of Drosophila guts following ingestion of sucrose (control), Erwinia carotovora carotovora 15, Pseudomonas entomophila, or Pseudomonas entomophila mutant gacA

2012-09-07 | E-MTAB-1200 | biostudies-arrayexpress

erwinia chrysanthemi treatments-BOS1 signaling pathway in Arabidopsis thaliana / Erwinia chrysanthemi interaction

Project description:ra06-04_bos1 - erwinia chrysanthemi treatments - BOS1 signaling pathway in Arabidopsis thaliana / Erwinia chrysanthemi interaction - Comparison of 4 transcriptoms : Wt Arabidopsis ecotype infected by Wt Erwinia chrysanthemi strain, bos1 Arabidopsis mutant infected by Wt Erwinia chrysanthemi strain, Wt Arabidopsis ecotype infected by BOS1-non inducing pelBC Erwinia chrysanthemi mutant, Wt Arabidopsis ecotype buffer inoculated. Experiments were repeated 3 times. Keywords: normal vs disease comparison

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Project description:ra05-02_erwinia - erwinia - Identification of Arabidopsis genes regulataed by Erwinia amylovora and of a subset of Arabiddopsis genes regulated by the type three secretion system of Erwinia amylovora. Keywords: normal vs disease comparison

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Pseudomonas entomophila

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erwinia chrysanthemi treatments-BOS1 signaling pathway in Arabidopsis thaliana / Erwinia chrysanthemi interaction

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Pseudomonas virulence factor controls expression of virulence genes in Pseudomonas entomophila

Project description:Quorum sensing is a communication strategy that bacteria use to collectively alter gene expression in response to cell density. Pathogens use quorum sensing systems to control activities vital to infection, such as the production of virulence factors and biofilm formation. The Pseudomonas virulence factor (pvf) gene cluster encodes a quorum sensing system (Pvf) that is present in over 500 strains of proteobacteria, including strains that infect a variety of plant and human hosts. We have shown that the Pvf quorum sensing system regulates the production of secreted proteins and small molecules in the insect pathogen Pseudomonas entomophila L48. Here, we identified genes that are likely regulated by Pvf using the model strain P. entomophila L48 which does not contain other known quorum sensing systems. Pvf regulated genes were identified through comparing the transcriptomes of wildtype P. entomophila and a pvf deletion mutant (ΔpvfA-D). We found that deletion of pvfA-D affected the expression of approximately 300 genes involved in virulence, the type VI secretion system, siderophore transport, and branched chain amino acid biosynthesis. Additionally, we identified seven putative biosynthetic gene clusters whose expression are reduced in ΔpvfA-D. Our results indicate that Pvf controls multiple virulence mechanisms in P. entomophila L48. Characterizing genes regulated by Pvf will aid understanding of host-pathogen interactions and development of anti-virulence strategies against P. entomophila and other pvf-containing strains.

2023-03-19 | GSE216056 | GEO

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