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HSP27 3A vs WT

ABSTRACT: human bronchial smooth muscle cells were infected with adenoviral constructs containing either the phosphorylation mutant form of HSP27 (HSP27-3A) or the wild type form (HSP27-WT) at 40 MOI for 4 days. All sets were stimulated with a cytokine mixture containing 10 ng/ml IL-1beta, TNF-alfa, and gamma-IFN for 20 hours prior to harvest. This series represents 4 separate preparations of cells and RNA, with each set being subjected to a dye flip and hybridized to 2 different slides. Keywords: parallel sample

ORGANISM(S): Homo sapiens

PROVIDER: GSE1292 | GEO | 2004/04/09

SECONDARY ACCESSION(S): PRJNA90209

REPOSITORIES: GEO

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HSP27 3A vs WT

Project description:human bronchial smooth muscle cells were infected with adenoviral constructs containing either the phosphorylation mutant form of HSP27 (HSP27-3A) or the wild type form (HSP27-WT) at 40 MOI for 4 days. All sets were stimulated with a cytokine mixture containing 10 ng/ml IL-1beta, TNF-alfa, and gamma-IFN for 20 hours prior to harvest. This series represents 4 separate preparations of cells and RNA, with each set being subjected to a dye flip and hybridized to 2 different slides.

2010-06-09 | E-GEOD-1292 | biostudies-arrayexpress

Adenovirus vs Mock Infection

Project description:human bronchial smooth muscle cells were either infected with empty adenoviral type 5 vector at 40 MOI for 4 days, or Mock infected. Each sample set was then stimulated with a mixture of 10ng/mL IL-1beta, TNF-alfa, and gamma-IFN for 20 hours prior to RNA harvest. RNA samples were split, and the series consists of 3 slides, 2 of which are dye flipped. Keywords: parallel sample

2004-04-09 | GSE1291 | GEO

Adenovirus vs Mock Infection

2010-06-09 | E-GEOD-1291 | biostudies-arrayexpress

Human pulmonary microvascular endothelial cells (HPMECs): Control siRNA vs HSP27 siRNA

Project description:Transcriptional profiling of HPMECs comparing Control siRNA treated with HSP27 siRNA Two-condition experiment, control siRNA vs HSP27 siRNA ; 3 controlsiRNA transfected replicates , 3HSP27 siRNA replicates. Replicates were pooled and hybridized to a single array.

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Hsp27 acts as a master molecular chaperones and plays an essential role in hepatocellular carcinoma metastasis

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HSP27 (Heat Shock Protein 27, HSPB1) overexpression effect on bleomycin-induced PF (Pulmonary fibrosis) mice model

Project description:To analyze the role of HSP27 in bleomycin (BLM)-induced pulmonary fibrosis in vivo, we used TG mice that conventionally overexpressed HSP27. Our previously reported that HSP27 plays an important role in pulmonary fibrosis. In mice overexpressing HSPB1, BLM-induced fibrotic regions and collagen deposits were increased compared with those in control BL6 mice. Following intratracheal treatment of BLM, the HSP25 TG mice showed an abundance of mononuclear and neutrophils cells in the alveoli, greater destruction of alveolar septa, intra-alveolar hyaline membrane formation, and a marked increase in collagen deposition compared with control BL6 mice.

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Hsp27 acts as a master molecular chaperones and plays an essential role in hepatocellular carcinoma metastasis

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Expression profiling, siRNA mediated knockdown of PPARbeta/delta and HSP27 it HeLa cells under IL1b stimulation

Project description:HeLa cells were depeleted for PPARbeta/delta or HSP27 and treated with IL1b

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Transcription profiling by array of castration-resistant prostate cancer PC-3 cells treated with Hsp27-siRNA or control siRNA to study the role of Heat shock protein (Hsp) 27 in splicing

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2015-06-30 | E-MTAB-1786 | biostudies-arrayexpress

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