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M6A modification in effector CD4+ T cells [m6A-miCLIP-seq]

ABSTRACT: GP61-primed effector CD4+ T cells were isolated from Ctrl or Mettl3-deficient SMARTA mice. Total RNAs were extracted with TRIzol reagent, and mRNAs were then isolated with Dynabeads® mRNA purification kit, followed by stardard m6A-miCLIP-SMARTer-seq with some modifications. Raw sequencing reads were aligned to the mouse genome (mm10) with BWA, and then m6A sites were determined.

ORGANISM(S): Mus musculus

PROVIDER: GSE129649 | GEO | 2021/01/19

REPOSITORIES: GEO

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