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DRIP-sequencing U2OS

ABSTRACT: Here, we identify that a human DNA helicase RTEL1, which is known to play a role in the maintenance of telomeres by interacting with proteins in the shelterin complex by resolving G4 quadruplexes, is essential for prevention of R-loop accumulation during replication and for MiDAS in mitosis at both CFSs and telomeres. Our findings indicate that RTEL1 plays a genome-wide role in preventing the collapse of replication forks at sites of G-quadruplexes and R-loop formation. Considering cancer cells rely on RTEL1 function to resolve the elevated transcription-replication conflicts caused by oncogene activation, RTEL1 is a potential drug target for cancer therapy.

ORGANISM(S): Homo sapiens

PROVIDER: GSE129907 | GEO | 2020/02/12

REPOSITORIES: GEO

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A cell cycle switch in TRF2 controls t-loop dynamics and DNA secondary structure resolution across the genome

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