Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

NanoString mRNA Profiling of Aptamer- and Antibody-Isolated CD8 Mock and CAR T Cells

ABSTRACT: The goal of this study was to see if isolating CD8 T cells from PBMCs with a DNA aptamer versus an antibody would have 1) any immediate transcriptional affect on freshly isolated T cells and 2) any downstream transcriptional affect on a manufactured CD19 CAR T cell product.

ORGANISM(S): Homo sapiens

PROVIDER: GSE130185 | GEO | 2019/05/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

NanoString mRNA Profiling of Aptamer- and Antibody-Isolated CD8 Mock and CAR T Cells

Project description:NanoString mRNA Profiling of Aptamer- and Antibody-Isolated CD8 Mock and CAR T Cells

| PRJNA534241 | ENA

ACE-Scan of the ATP DNA aptamer on various density Gen2 DNA microarrays

Project description:The experiments were carried out to map the ligand binding landscape of various DNA and RNA duplexed aptamer families. Duplexed Aptamer (DA) constructs were engineered from (i) natural and synthetic DNA and RNA aptamers and (i) synthetic oligonucleotide aptamer-complementary elements synthesized on custom DNA microarrays. The aptamers tested consist of the ATP DNA aptamer, the ATP RNA aptamer, the cocaine DNA aptamer, the human alpha-thrombin DNA aptamer, and the natural add riboswitch aptamer from the pathogenic bacteria Vibrio vulnificus. Each duplexed aptamer family consists of 1000's of synthetic constructs, each formed by hybridizing the aptamer with an aptamer-complementary element (ACE) - here, ACEs consisted of various DNA oligonucleotides synthesized as a custom DNA microarray.

2017-12-20 | E-MTAB-6375 | biostudies-arrayexpress

ACE-Scan of the cocaine DNA aptamer on 10^12 Gen1 DNA microarray

2017-12-20 | E-MTAB-6377 | biostudies-arrayexpress

ACE-Scan of the ATP RNA aptamer on 10^12 Gen1 DNA microarray

2017-12-20 | E-MTAB-6376 | biostudies-arrayexpress

ACE-Scan of the ATP DNA aptamer on 10^12 density Gen1 DNA microarray

2017-12-20 | E-MTAB-6374 | biostudies-arrayexpress

ACE-Scan of the thrombin DNA aptamer on 10^10 Gen1 DNA microarrays with sodium and potassium buffers in two colors.

2017-12-20 | E-MTAB-6378 | biostudies-arrayexpress

ACE-Scan of the natural add riboswitch aptamer on 10^10 Gen1 DNA microarrays with varying buffer temperatures and Mg2+ cation concentrations

2017-12-20 | E-MTAB-6380 | biostudies-arrayexpress

Aptamer Affinity Mass Spectrometry

Project description:Antibody-based affinity purification of macromolecular complexes has revolutionized the study of protein-protein interactions. Here, we present AptA-MS (Aptamer Affinity – Mass Spectrometry), a robust strategy using a specific, high-affinity RNA aptamer against GFP to identify novel interactors of a GFP-tagged protein using high resolution MS. AptA-MS offers a high signal-to-noise ratio due to the absence of immunoprecipitation-derived contaminants and allows the identification of post-translational modifications without the need for modification-specific enrichments.

2020-06-17 | PXD015620 | Pride

A sputum bioassay for airway eosinophilia using an eosinophil peroxidase aptamer

Project description:We genearted a DNA aptamer against EPX via SELEX protocol using a random DNA library and developed an assay to monitor EPX in patients spitum samples After 15 rounds of selection, the aptamer sequences were obtained by high throughput sequencing

2022-09-25 | GSE213923 | GEO

T1 aptamer targets intratumoral myeloid cells with immunosuppression functions

Project description:Intratumorally, immunosuppressive myeloid subsets are difficult to distinguish from mature, non-suppressive counterparts. T1 aptamer specifically targets the former subset and was used here to isolated the subsets for transcriptomic profiling.

2021-11-24 | GSE185974 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data