Transcriptomics

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Transcriptional profiling of Brucella ovis strains cultivated in the presence of high (5%) and low (0.04%) CO2


ABSTRACT: Wild-type Brucella ovis ATCC 25840 requires the addition of 5% CO2 to the atmosphere to grow on either nutrient agar plates or in liquid broth culture. The goal of this study was to measure the transcriptional response of two Brucella ovis strains under high (5%) and low (0.04%) CO2. The two strains assayed were 1) wild-type and 2) a spontaneous mutant that can be cultivated in standard atmospheric levels of CO2 (~0.04%). Wild-type B. ovis harbors a single nucleotide insertion at the 3' end of a beta carbonic anhydrase gene, bcaA, which renders it non-functional. The spontaneous mutant lost this nucleotide insertion, which restored the consensus reading frame and results in a functional BcaA protein.

ORGANISM(S): Brucella ovis

PROVIDER: GSE130678 | GEO | 2019/05/04

REPOSITORIES: GEO

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