Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation

ABSTRACT: We investigated the transcriptional consequences of Chd7 and Runx1 loss in 416B cells, a myeloid progenitor cell line. We engineered the cell line to express Cas9 protein and targeted endogenous Chd7 and Runx1 loci with sgRNAs introduced with lentiviral constructs. We read out the transcriptomes of control and perturbed cells using RNA-Seq following an established SMART-Seq2 protocol (Picelli et al. 2014).

ORGANISM(S): Mus musculus

PROVIDER: GSE130719 | GEO | 2022/04/26

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation [ATAC-seq]

Project description:Hematopoietic stem and progenitor cell (HSPC) formation and lineage differentiation involve gene expression programs orchestrated by transcription factors and epigenetic regulators. Knockdown of the chromatin remodeler chromodomain-helicase-DNA-binding protein 7 (CHD7) expanded phenotypic HSPCs, erythroid, and myeloid lineages in zebrafish and mouse embryos. CHD7 acts to suppress hematopoietic differentiation in a cell autonomous manner in the embryo and adult. CHD7 chromatin immunoprecipitation in human CD34+ and mouse HSPCs revealed enrichment of binding motifs for hematopoietic transcription factors including Runx1 and GATA factors, and decreased Runx1 occupancy correlated with loss of CHD7 occupancy. CHD7 physically interacts with Runx1 and suppresses Runx1-induced expansion of HSPCs during development, providing both physical and genetic evidence for the Runx1-CHD7 interaction. CHD7 modulates Runx1 activity to provide proper timing and function of HSPCs as they emerge during hematopoietic development or mature in adults, representing a distinct and evolutionarily conserved control mechanism to ensure accurate hematopoietic lineage differentiation.

2023-01-01 | GSE130280 | GEO

CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation [RNA-seq]

2023-01-01 | GSE84128 | GEO

CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation

Project description:Hematopoietic stem and progenitor cell (HSPC) formation and lineage differentiation involve gene expression programs orchestrated by transcription factors and epigenetic regulators. Knockdown of the chromatin remodeler chromodomain-helicase-DNA-binding protein 7 (CHD7) expanded phenotypic HSPCs, erythroid, and myeloid lineages in zebrafish and mouse embryos. CHD7 acts to suppress hematopoietic differentiation in a cell autonomous manner in the embryo and adult. We performed ChIP sequencing to determine the genomic localization of CHD7 in human CD34+ and mouse HSPCs, revealing enrichment of binding motifs for hematopoietic transcription factors including Runx1 and GATA factors. CHD7 occupied regions are transcriptionally active and are near genes with hematopoietic function. Decreased Runx1 occupancy correlated with loss of CHD7 occupancy. Together, the physical and genetic interaction data support a model in which CHD7 interacts with and modulates Runx1 activity to provide proper timing and function of HSPCs as they emerge during hematopoietic development or mature in adults. This represents a distinct and evolutionarily conserved control mechanism to ensure accurate hematopoietic lineage differentiation.

2019-07-27 | GSE83956 | GEO

CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation [G1ER ChIP-seq]

2023-01-01 | GSE84129 | GEO

CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation [CD34 ChIP-seq]

2023-12-01 | GSE84130 | GEO

CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation

Project description:CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation

| PRJNA328122 | ENA

CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation

Project description:CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation

| PRJNA541162 | ENA

CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation [ATAC-seq]

Project description:CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation [ATAC-seq]

| PRJNA534517 | ENA

Single cell RNA-sequencing of human tonsil Innate lymphoid cells (ILCs)

Project description:Single cell RNA-sequencing of human tonsil Innate lymphoid cells (ILCs) from three independent tonsil donors. Sequencing libraries were prepared from FACS sorted individual ILCs with the Smart-Seq2 protocol (Picelli et al. Nature Methods 2013)

2016-01-27 | E-GEOD-70580 | biostudies-arrayexpress

Expression Profile of CHD7-Deficient Murine Long-term Hematopoietic Stem Cells

2018-07-01 | GSE84136 | GEO