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Genome-wide maps of chromatin state (centromeric histone H3 variant CENP-A/Cnp1) in clr4∆ and the transformants from the deletion of clr4 in mhf2∆ (cen2_inactive) or mhf2+ (cen1_inactive).

ABSTRACT: We report the high-throughput profiling of histone variant CNEP-A/Cnp1 in fission yeast Schizosaccharomyces pombe. By obtaining 1-10 ng immunoprecipitated DNA, we generated genome-wide CENP-A/Cnp1 maps of clr4∆ and the transformants from the deletion of clr4 in mhf2∆ (cen2_inactive) or mhf2+ (cen1_inactive) in fission yeast. We find that heterochromatin is not required for the induction of centromere inactivation in mhf2∆ and the maintenance of neocentromere in mhf2+.

ORGANISM(S): Schizosaccharomyces pombe

PROVIDER: GSE131226 | GEO | 2019/09/28

REPOSITORIES: GEO

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