Transcriptomics

Dataset Information

0

A large peptidome dataset improves HLA class I epitope prediction across most of the human population


ABSTRACT: Prediction of HLA epitopes is important for the development of cancer immunotherapies and vaccines. However, current prediction algorithms have limited predictive power, in part because they were not trained on high-quality epitope datasets covering a broad range of HLA alleles. To enable prediction of endogenous HLA class I-associated peptides across a large fraction of the human population, we used mass spectrometry to profile >185,000 peptides eluted from 95 HLA-A, -B, -C and -G mono-allelic cell lines. We identified canonical peptide motifs per HLA allele, unique and shared binding submotifs across alleles and distinct motifs associated with different peptide lengths. By integrating these data with transcript abundance and peptide processing, we developed HLAthena, providing allele-and-length-specific and pan-allele-pan-length prediction models for endogenous peptide presentation. These models predicted endogenous HLA class I-associated ligands with 1.5-fold improvement in positive predictive value compared with existing tools and correctly identified >75% of HLA-bound peptides that were observed experimentally in 11 patient-derived tumor cell lines.

ORGANISM(S): Homo sapiens

PROVIDER: GSE131267 | GEO | 2019/12/16

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2019-08-06 | MSV000084172 | MassIVE
2019-10-09 | MSV000084442 | MassIVE
2017-02-21 | GSE93315 | GEO
2021-09-17 | PXD028088 | Pride
2019-12-18 | PXD013831 | Pride
2024-01-26 | PXD045796 | Pride
2017-02-01 | MSV000080527 | MassIVE
2018-07-21 | MSV000082648 | MassIVE
2019-06-18 | MSV000083991 | MassIVE
2023-01-13 | PXD038387 | Pride