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Secreted HLA Fc-Fusion Profiles Immunopeptidome in Hypoxic PDAC and Cellular Senescence

ABSTRACT: Here, we describe a secreted HLA (sHLA) Fc-fusion construct for simple single HLA allele profiling in hypoxic pancreatic ductal adenocarcinoma (PDAC) and cellular senescence. This method streamlines sample preparation, enables temporal control, and provides allele-restricted target identification. Over 30,000 unique HLA-associated peptides were identified across two different HLA alleles and seven cell lines, with ~9,300 peptides newly discovered. The sHLA Fc-fusion capture technology holds potential to expedite immunopeptidomics and advance therapeutic interest in peptide-HLA complexes.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Lisa Kirkemo

LAB HEAD: Jim Wells

PROVIDER: PXD045796 | Pride | 2024-01-26

REPOSITORIES: Pride

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			Action	DRS
	210210_MiaPaca2_sHLA_Hypoxia_1_Slot2-5_1_873.d.zip	Other
	210210_MiaPaca2_sHLA_Hypoxia_2_Slot2-6_1_874.d.zip	Other
	210210_MiaPaca2_sHLA_Normoxia_1_Slot2-3_1_871.d.zip	Other
	210210_MiaPaca2_sHLA_Normoxia_2_Slot2-4_1_872.d.zip	Other
	210212_B721.221_35.01_2_Slot2-2_1_893.d.zip	Other

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Secreted HLA-Fc fusion profiles immunopeptidome in hypoxic PDAC and cellular senescence.

Rettko Nicholas J NJ Kirkemo Lisa L LL Wells James A JA

PNAS nexus 20231214 12

Human leukocyte antigens (HLA) present peptides largely from intracellular proteins on cell surfaces. As these complexes can serve as biomarkers in disease, proper identification of peptides derived from disease-associated antigens and the corresponding presenting HLA is important for the design and execution of therapeutic strategies. Yet, current mass spectrometry methods for immunopeptidomic profiling require large and complex sample inputs, hindering the study of certain disease phenotypes a ...[more]

PMID: 38099269

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