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Leukocyte - endothelial cell interaction in infections: the role of IL1, TNF and IFN pathways

ABSTRACT: Here we comprehensively characterized the transcriptomic responses of human leukocytes (PBMC) and EC to Gram negative-bacteria, Gram positive-bacteria, and fungi by RNAseq. We showed that the common response of leukocytes to various pathogens converges on EC activation. By exposing EC to leukocyte-released mediators, with or without the presence of IL1RA and TNFα antibody, we identified specific roles for IL1 and TNFα in driving the majority of, but not exclusively, endothelial activation. Furthermore, interferon pathways are strongly induced in EC by leukocyte-released mediators, but independently from IL1 and TNFα. Our study, therefore reveals a role for IFN, together with IL1 and TNFα signaling, in mediating leukocyte-endothelial interaction in infections

ORGANISM(S): Homo sapiens

PROVIDER: GSE131590 | GEO | 2021/05/11

REPOSITORIES: GEO

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