Project description:To study the dynamics of acid adaptation in E.coli at pH5.5 as opposed to that at pH7, samples were collected from a steady-state system for transcriptomics. Keywords: time course
Project description:To study the dynamics of acid adaptation in E.coli at pH5.5 as opposed to that at pH7, samples were collected from a steady-state system for transcriptomics. Keywords: time course
Project description:To study the dynamics of acid adaptation in E.coli at pH5.5 as opposed to that at pH7, samples were collected from a steady-state system for transcriptomics. Keywords: time course Single channel hybridisation were carried out using cy5 dye. Samples were collected from steady state system at pH7 and pH5.5 for 2.5h at 0min, 10min, 30min, 1h, 1.5h, 2h, 2.5h
Project description:To study the dynamics of acid adaptation in E.coli at pH5.5 as opposed to that at pH7, samples were collected from a steady-state system for transcriptomics. Keywords: time course Single channel hybridisation were carried out using cy5 dye. Samples were collected from steady state system at pH7 and pH5.5 for 1hr every 5min.
Project description:To study the dynamics of acid adaptation in E.coli at pH5.5 as opposed to that at pH7, samples were collected from a steady-state system for transcriptomics. Keywords: time course
Project description:To study the dynamics of acid adaptation in E.coli at pH5.5 as opposed to that at pH7, samples were collected from a steady-state system for transcriptomics. Keywords: time course Single channel hybridisation were carried out using cy5 dye. Samples were collected from steady state system at pH7 and pH5.5 for 2.5h at 0min, 10min, 30min, 1h, 1.5h, 2h, 2.5h
Project description:This study explores the effects of prolonged stationary phase, on whole genome expression of Escherichia coli. In summary these results imply that prolonged stationary phase cells have shown less growth rate compared to fresh cells due to the low abundance of transcripts for translation initiation factor infC, and for all the seven rrn operons of 5S, 16S, 23S ribosomal RNA which further delayed in protein synthesis leading to low biomass in prolonged stationary phase cells.
Project description:To study the dynamics of acid adaptation in E.coli Keio collection mutant library at pH7 and pH5.5 after 15 minutes of adaptation Keywords: Single channel hybridisation were carried out using cy5 dye. Samples were collected from steady state system at pH7 and at pH5.5 after 15 minutes of adaptation
Project description:To study the dynamics of acid adaptation in E.coli Keio collection mutant library at pH7 and pH5.5 after 15 minutes of adaptation Keywords: Single channel hybridisation were carried out using cy5 dye.