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Rinf regulates pluripotency network and Tet enzymes in embryonic stem cells (ESCs)

ABSTRACT: In this study: (1) We have mapped the genome wide binding distribution and enrichment of Rinf/CXXC5 at genes and regulatory regions in mouse ESCs by ChIP-seq using a specific antibody against Rinf. (2) We have examined the role of Rinf in regulation of ESC gene expression programs by performing transcriptomic analysis of Rinf wild type and knockout ESCs by RNA-seq to identify differentially expressed genes. (3) We have investigated the role of Rinf in differentiation and lineage specification programs of ESCs by analyzing the transcriptomic profile of wild type and Rinf–/– ESCs during differentiation to embroyoid bodies (EBs) at three time points (day 0, 3, 6).

ORGANISM(S): Mus musculus

PROVIDER: GSE132025 | GEO | 2019/08/05

REPOSITORIES: GEO

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Loss of Tet enzymes compromises proper differentiation of embryonic stem cells

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