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PocR regulates glycerol reduction, assembly of metabolosomes and vitamin B12 biosynthesis in Lactobacillus reuteri F275

ABSTRACT: Lactobacillus reuteri has been shown to encode a vitamin B12 biosynthesis pathway that is phylogenetically related to the one present in some representatives of gamma-proteobacteria such as Salmonella and Yersinia. Here we present evidence supporting that the similarities between these otherwise unrelated organisms extend to their regulatory mechanisms. Keywords: cell type comparison

ORGANISM(S): Limosilactobacillus reuteri subsp. reuteri F275

PROVIDER: GSE13289 | GEO | 2008/10/31

SECONDARY ACCESSION(S): PRJNA109613

REPOSITORIES: GEO

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PocR regulates glycerol reduction, assembly of metabolosomes and vitamin B12 biosynthesis in Lactobacillus reuteri F275

Project description:Lactobacillus reuteri has been shown to encode a vitamin B12 biosynthesis pathway that is phylogenetically related to the one present in some representatives of gamma-proteobacteria such as Salmonella and Yersinia. Here we present evidence supporting that the similarities between these otherwise unrelated organisms extend to their regulatory mechanisms. Keywords: cell type comparison Loop design

2010-05-17 | E-GEOD-13289 | biostudies-arrayexpress

Effect of amino acid availability on vitamin B12 production in Lactobacillus reuteri

Project description:Recent functional genomics and genome-scale modeling approaches indicated that B12 production in Lactobacillus reuteri could be improved by medium optimization. Here we show that a series of systematic single amino acid omissions could significantly modulate the production of B12 from nearly undetectable levels (by isoleucine omission) to 20-fold higher than previously reported through omission of cysteine. We analyzed by cDNA microarray experiments the transcriptional response of L. reuteri to the medium lacking cysteine. These results supported the observed high B12 production and provided new avenues for future improvement of production of vitamin B12. Keywords: cell type comparison

2008-10-31 | GSE13391 | GEO

Effect of amino acid availability on vitamin B12 production in Lactobacillus reuteri

2010-05-17 | E-GEOD-13391 | biostudies-arrayexpress

The impact of glycerol on the metabolism of Lactobacillus reuteri - Main experiment

Project description:Lactobacillus reuteri is a heterofermentative lactic acid bacterium best known for its ability to co-ferment glucose and glycerol. Its genome sequence has recently been deduced enabling the implementation of genome-wide analysis. In this study we developed a dedicated cDNA microarray platform and a genome-scale metabolic network model of L. reuteri and use them to revisit the co-fermentation of glucose and glycerol. The model was used to simulate experimental conditions and to visualize and integrate experimental data in particular the global transcriptional response of L. reuteri to the presence of glycerol. We show how the presence of glycerol affects cell physiology and triggers specific regulatory mechanisms allowing simultaneously a better yield and more efficient biomass formation. Furthermore we were able to predict and demonstrate for this well-studied condition the involvement of previously unsuspected metabolic pathways for instance related to amino acids and vitamins. These could be used as leads in future studies aiming at the increased production of industrially relevant compounds such as vitamin B12 or 1 3- propanediol. Keywords: cell type comparison

2008-09-15 | GSE11861 | GEO

The impact of glycerol on the metabolism of Lactobacillus reuteri - Exploratory experiment

2008-09-15 | GSE11860 | GEO

The impact of glycerol on the metabolism of Lactobacillus reuteri - Exploratory experiment

2010-05-20 | E-GEOD-11860 | biostudies-arrayexpress

The impact of glycerol on the metabolism of Lactobacillus reuteri - Main experiment

2008-09-15 | E-GEOD-11861 | biostudies-arrayexpress

The impact of glycerol on the metabolism of Lactobacillus reuteri

Project description:This SuperSeries is composed of the following subset Series: GSE11860: The impact of glycerol on the metabolism of Lactobacillus reuteri - Exploratory experiment GSE11861: The impact of glycerol on the metabolism of Lactobacillus reuteri - Main experiment Refer to individual Series

2010-05-20 | E-GEOD-11885 | biostudies-arrayexpress

Lactobacillus reuteri strains at different growth phases

Project description:This SuperSeries is composed of the following subset Series: GSE24415: Comparison of gene expression of L. reuteri ATCC PTA 6475 at different growth phase in a LDMIII GSE24570: Comparison of gene expression of L. reuteri ATCC 55730 at different growth phase in LDMIII medium Refer to individual Series

2011-01-15 | E-GEOD-24572 | biostudies-arrayexpress

Lactobacillus reuteri ATCC 55730: Before vs after exposure to 0.5% bile.

Project description:Transcriptional profiling of Lactobacillus reuteri ATCC 55730 mid-log cultures before vs after exposure to 0.5% bovine bile (oxgall). Two sets of array experiments were performed. One set compared the expression profiles of L. reuteri ATCC 55730 cells before bile exposure vs cells that had been exposed to 0.5% bile for 15 minutes (bile shock). The other set compared the expression profiles of L. reuteri ATCC 55730 cells before bile exposure vs cells that had begun growing again in the presence of 0.5% bile (bile adaptation). Keywords: Stress response

2008-02-05 | GSE10155 | GEO

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