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Effect of amino acid availability on vitamin B12 production in Lactobacillus reuteri

ABSTRACT: Recent functional genomics and genome-scale modeling approaches indicated that B12 production in Lactobacillus reuteri could be improved by medium optimization. Here we show that a series of systematic single amino acid omissions could significantly modulate the production of B12 from nearly undetectable levels (by isoleucine omission) to 20-fold higher than previously reported through omission of cysteine. We analyzed by cDNA microarray experiments the transcriptional response of L. reuteri to the medium lacking cysteine. These results supported the observed high B12 production and provided new avenues for future improvement of production of vitamin B12. Keywords: cell type comparison

ORGANISM(S): Limosilactobacillus reuteri subsp. reuteri F275

PROVIDER: GSE13391 | GEO | 2008/10/31

SECONDARY ACCESSION(S): PRJNA109819

REPOSITORIES: GEO

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Effect of amino acid availability on vitamin B12 production in Lactobacillus reuteri

Project description:Recent functional genomics and genome-scale modeling approaches indicated that B12 production in Lactobacillus reuteri could be improved by medium optimization. Here we show that a series of systematic single amino acid omissions could significantly modulate the production of B12 from nearly undetectable levels (by isoleucine omission) to 20-fold higher than previously reported through omission of cysteine. We analyzed by cDNA microarray experiments the transcriptional response of L. reuteri to the medium lacking cysteine. These results supported the observed high B12 production and provided new avenues for future improvement of production of vitamin B12. Keywords: cell type comparison loop design

2010-05-17 | E-GEOD-13391 | biostudies-arrayexpress

PocR regulates glycerol reduction, assembly of metabolosomes and vitamin B12 biosynthesis in Lactobacillus reuteri F275

Project description:Lactobacillus reuteri has been shown to encode a vitamin B12 biosynthesis pathway that is phylogenetically related to the one present in some representatives of gamma-proteobacteria such as Salmonella and Yersinia. Here we present evidence supporting that the similarities between these otherwise unrelated organisms extend to their regulatory mechanisms. Keywords: cell type comparison

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PocR regulates glycerol reduction, assembly of metabolosomes and vitamin B12 biosynthesis in Lactobacillus reuteri F275

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Limosilactobacillus reuteri subsp. reuteri F275

Project description:Effect of amino acid availability on vitamin B12 production in Lactobacillus reuteri

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The impact of glycerol on the metabolism of Lactobacillus reuteri - Exploratory experiment

Project description:Lactobacillus reuteri is a heterofermentative lactic acid bacterium best known for its ability to co-ferment glucose and glycerol. Its genome sequence has recently been deduced enabling the implementation of genome-wide analysis. In this study we developed a dedicated cDNA microarray platform and a genome-scale metabolic network model of L. reuteri and use them to revisit the co-fermentation of glucose and glycerol. The model was used to simulate experimental conditions and to visualize and integrate experimental data in particular the global transcriptional response of L. reuteri to the presence of glycerol. We show how the presence of glycerol affects cell physiology and triggers specific regulatory mechanisms allowing simultaneously a better yield and more efficient biomass formation. Furthermore we were able to predict and demonstrate for this well-studied condition the involvement of previously unsuspected metabolic pathways for instance related to amino acids and vitamins. These could be used as leads in future studies aiming at the increased production of industrially relevant compounds such as vitamin B12 or 1 3- propanediol. Keywords: cell type comparison Dye swap

2010-05-20 | E-GEOD-11860 | biostudies-arrayexpress

The impact of glycerol on the metabolism of Lactobacillus reuteri - Main experiment

2008-09-15 | E-GEOD-11861 | biostudies-arrayexpress

The impact of glycerol on the metabolism of Lactobacillus reuteri - Main experiment

2008-09-15 | GSE11861 | GEO

The impact of glycerol on the metabolism of Lactobacillus reuteri - Exploratory experiment

2008-09-15 | GSE11860 | GEO

Global transcriptome analysis of Lactobacillus reuteri in sourdough fermentation.

Project description:In this study, we investigated the transcriptional response of the human isolate L. reuteri ATCC 55730 during sourdough fermentation by using whole-genome microarrays. Significant changes of mRNA levels were found for 101 genes involved in diverse cellular processes, e.g., carbohydrate and energy metabolism, cell envelope biosynthesis, exopolysaccharide production, stress responses, signal transduction and cobalamin biosynthesis. Our results evidence extensive changes of the organism’s gene expression to the growth in sourdough as compared to the growth in chemically defined medium, and thus allowed us to uncover pathways involved in the adaptation of L. reuteri to the ecological niche of sourdough. An impact of several genes of L. reuteri for effective growth in sourdough was shown by implementation of mutant strains in sourdough fermentation. This study contributes to the understanding of the molecular fundamentals of L. reuteri’s ecological competitiveness, and provides a basis for further exploration of genetic traits involved in adaptation to the food and/or intestinal environment. Keywords: environment-specific gene expression, sourdough fermentation, chemically defined medium, dye swap

2008-02-13 | GSE10495 | GEO

Comparison of gene expression of L. reuteri ATCC PTA 6475 and L. reuteri 6475 PocR insertant

Project description:Comparison of gene expression between L. reuteri ATCC PTA 6475 and PocR mutant grown in semi-defined medium after 24h of growth at 37C in anaerobic condition

2010-12-10 | GSE22926 | GEO

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