Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome changes of liver from mice overexpressing Chrna4

ABSTRACT: We injected adenovirus overexpressing Chrna4 as well as vehicle via tail vein (1× 10^9 pfu per mouse). Mice were fed with high-fat diet at the same time. After two weeks from virius injection, mice were sacrificed and samples were collected

ORGANISM(S): Mus musculus

PROVIDER: GSE133944 | GEO | 2022/07/08

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression data from PDK4 overexpressing (Ad-PDK4 infected) and control (Ad-control infected) mouse liver

Project description:We identified PDK4 as a gene with adaptive transcriptional response to chemical stress. Although PDK4 is an energy resource regulator induced by starvation, expression of other fasting-inducible genes was unaffected, indicating additional physiological role of PDK4 for liver adaptation to the chemical stress. We used microarrays to determine genes with altered transcriptional level by PDK4 overexpression. Mice were infected with Ad-control (empty adenovirus vector) or Ad-PDK4 (PDK4 overexpressing adenovirus vector) at a dose of 10^9 PFU/mouse by tail vein injection. 3 days after the infection, mice were sacrificed for RNA preparation from the liver. Ad-control infected = 4, Ad-PDK4 infected = 3. Specimens from mice of each group were pooled, and 10 ug RNA from each pool was used for cRNA synthesis.

2010-12-25 | E-GEOD-22534 | biostudies-arrayexpress

Cachexia-inducible Transforming growth factor-beta1 stimulated Clone-22 D4 controls acute hepatic lipid homeostasis.

Project description:To explore the molecular basis for TSC22D4 function in hepatic lipid homeostasis in vivo TSC22D4 was knocked down in the mouse liver using adenovirus and performed genome wide expression analysis. 1x10^9 plaque-forming units (pfu) per recombinant virus were administered via tail vein injection. Mice (C57Bl/6) were sacrificed 7 days after infection. Hepatic RNA was isolated and used for whole genome expression analysis.

2012-09-01 | E-GEOD-33324 | biostudies-arrayexpress

Neutrophil depletion experiment in ischemia reperfusion mouse model

Project description:To elucidate the role of neutrophils in the molecular alterations observed after I/R, we performed an experiment of I/R in neutrophil-depleted mice. Neutrophil depletion was performed by anti-Ly6G injection and IgG was used as vehicle. We have performed an unbiased, open-search analysis of the oxidative PTMs and quantitative multiplexed proteomics that take place in the mouse heart tissue at 24h after I/R. Neutrophil depletion was performed in 3 mice by Ly6G injection 24h and 48 h prior to I/R and sacrificed 24 hours after reperfusion. A second group consisting on 3 mice that were injected with IgG antibody as vehicle, and subjected to I/R and sacrificed 24h after reperfusion. A third group of 3 mice with no injection were also sacrificed 24h after reperfusion. 12 additional mice from the three previous groups were sacrificed without I/R intervention: control without injection (n=6), IgG injection (n=3) and Ly6G injection (n=3). Whole hearts tissue was collected for proteomics evaluation.

2024-05-24 | PXD045756 | Pride

Med1 overexpression induces cell proliferation

Project description:Med1 overexpression leads to induction of a wide spectrum of genes. Adenovirally-driven overexpression of Med1 in mouse liver stimulates hepatocyte DNA synthesis with enhanced expression of DNA replication, cell cycle control and liver specific genes as observed at day 3 and day 5 post injection. Med1 gene is amplified in a number of cancers so in this study we tested the hypothesis that Med1 by itself has the capacity to induce cell proliferation. Analysis of the Med1- induced gene expression showed a robust induction of a wide spectrum of genes involved in hepatocellular proliferation. Med1 floxed mice (Med1fl/fl) were injected with Ad-His-Med1 (adenovirus expressing Med1 gene) via tail vein and killed 3 or 5 days after injection. Ad-LacZ (adenovirus expression beta-galactosidase gene) injected mouse liver served as control. Total RNA were isolated from the liver and subjected to the microarray.

2013-07-18 | E-GEOD-48950 | biostudies-arrayexpress

Liver transcriptome analysis of mice with ASirs2 suppression

Project description:Purpose: We show that the Irs2 locus, a critical regulator of insulin actions, encodes an antisense transcript ASIrs2, whose expression increased after refeeding in liver, reciprocally to that of Irs2. In order to elucidate the functions of ASIrs2, the liver transcriptome was analyzed under ASIrs2 suppression in wild type mice after refeeding. Methods: C57BL/6 mice were injected with AAV encoding shRNA for ASIrs2 via the via tail vein at 8 weeks of age, and the mice were sacrificed for organ collection after overnight fasting and subsequently 6 hours of refeeding on the 27th or 28th day after AAV injection.

2022-01-06 | GSE188428 | GEO

lung metastasis 100% vs 0%

Project description:Lung metastatic potential of the 29 HNSCC was examined with tail vein metastatic assay as described previously. 1 ×10E6 HNSCC cells in a volume of 200 µL were injected into the lateral tail vein using a 30 gauge needle. Eight to 10 mice were prepared for each cell line. Mice were euthanized using carbon dioxide asphyxiation when they lost more than 15% of their pre-injection body weight or at 90 days after cell injection. Necropsy was performed, and lung was harvested. The lungs were then evaluated for the presence of lung metastases and also weighed.

2017-02-09 | GSE67275 | GEO

Bmal1-ethanol based diet and liver injury

Project description:The mice were injected via tail vein with either shBmal1 adenovirus or shLacz adenocirus as control (n=5 for each group), following 10 days of 5% ethanol diet feeding. Then the mice were dissected and liver tissues were flash freezed. 25mg liver tissues from each mouse of the same group were pooled.

2017-06-05 | ST000674 | MetabolomicsWorkbench

Liver fibrosis model of hepatocyte-specific FOXA2 knockout mice

Project description:Liver firbrosis model of hepatocyte-specific FOXA2 knockout mice. Adeno-associated virus AAV8-TBG-Control or AAV8-TBG-Cre was injected via the tail vein of FOXA2flox/flox (FOXA2f/f) mice 2 weeks prior to CCl4 administration. Hepatic fibrosis was induced by injection of CCl4 twice per week for 4 weeks.

2017-04-04 | GSE97344 | GEO

Cell line xenograft experiment

Project description:Pediatric ALL cell line 380 was injected via tail vein into NSG mice. Triplicate 380 cell samples were also prepared at the timepoint of injection and stored at -80 oC for comparative analysis with CDXs. Mice were euthanized at onset of leukemia symptoms and spleen, bone marrow and liver samples were collected for proteomics analysis.

2021-01-20 | MSV000086727 | MassIVE

Metabolomics Workbench ST000674 - GNPS - Bmal1-ethanol based diet and liver injury

2018-08-02 | MSV000082704 | GNPS