Transcriptomics

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Bulk RNA-seq of mouse cells from the dorsal cerebral cortex during its formation, with knocked-out Ski gene


ABSTRACT: From mice treated with knocked-out Ski gene. Dorsal cortices from embryonic day E11.5 to E18.5 were micro-dissected and dissociated into single cell suspensions using Papain and Ovo-mucoid mix. Cells were washed with L15 medium and FAC-sorted for GFP positive NSCs using FACSariaIII (BD Biosciences) derived from Hes5::GFP transgenic embryos for NSCs and Tbr2::GFP transgenic embryos for BPs and NBNs.

ORGANISM(S): Mus musculus

PROVIDER: GSE134690 | GEO | 2020/12/31

REPOSITORIES: GEO

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