Similar Datasets
Project description:Identification of stress responsive miRNAs in Arabidopsis under altered GSH condition
| PRJNA556655 | ENA
Project description:GSH, being a versatile molecule, is actively involved in various bilogical processe of plant system. Our previous studies identifies an active role of GSH in plant defense signaling network. Here, we used microarray under GSH treated condition to obtain a global expression profiling under this altered GSH conditions.
2013-04-02 | GSE45677 | GEO
Project description:GSH, being a versatile molecule, is actively involved in various bilogical processe of plant system. Our previous studies identifies an active role of GSH in plant defense signaling network. Here, we used microarray under GSH treated condition to obtain a global expression profiling under this altered GSH conditions. We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process. A.thaliana, much accalimed model system of plant biology and being fully sequenced, we used this system to explore the specific relation of GSH with metabolic processes, phisiological conditions, etc.
2013-04-02 | E-GEOD-45677 | biostudies-arrayexpress
Project description:Glutathione (GSH) is a tripeptide thiol abundantly present in all eukaryotes1. As the major site of oxidative metabolism, mitochondria must maintain sufficient levels of GSH in order to perform key protective and biosynthetic functions. GSH is exclusively synthesized in the cytosol, yet how it enters mitochondria and the molecular machinery involved in mitochondrial GSH import remain elusive. Here, using mass-spectrometry approaches, we identify SLC25A39, a mitochondrial membrane carrier of unknown function, to mediate GSH transport into mitochondria. SLC25A39 loss reduces mitochondrial GSH import and abundance, without impacting whole cell GSH levels.
2021-11-15 | PXD027673 | Pride
Project description:Arabidopsis wild type and atglr3.3 mutant seedlings were cultured on 0.7% (w/v) agar medium containing half strength of MS basic salt with 1% sucrose (w/v), 5mM MES, pH 5.7. 10-day-old seedlings were equilibrated in the CK buffer for 24 hours. Then, these seedlings were either treated with the CK buffer or the CK buffer containing 100 ?M GSH and 100 ?M Glu for 1 hour, respectively.
2013-09-30 | E-MEXP-3885 | biostudies-arrayexpress
Project description:The essential thiol antioxidant, glutathione (GSH) is recruited into the nucleus of mammalian cells early in cell proliferation, suggesting a key role of the nuclear thiol pool in cell cycle regulation. However, the functions of nuclear GSH (GSHn) and its integration with the cytoplasmic GSH (GSHc) pools in whole cell redox homeostasis and signaling are unknown. Here we show that GSH is recruited into the nucleus early in cell proliferation in Arabidopsis thaliana, confirming the requirement for localization of GSH in the nucleus as a universal feature of cell cycle regulation. GSH accumulation in the nucleus was triggered by treatments that synchronize cells at G1/S as identified by flow cytometry and marker transcripts. Significant decreases in transcripts associated with oxidative signaling and stress tolerance occurred when GSH was localized in the nucleus. Increases in GSH1 and GSH2 transcripts accompanied the large increase in total cellular GSH observed during cell proliferation, but only GSH2 was differentially expressed in cells with high GSHn relative to those with an even intracellular distribution of GSH. Of the 7 Bcl-2 associated (BAG) genes in A. thaliana, only the nuclear-localized BAG 6 was differentially expressed in cells with high GSHn compared to GSHc. We conclude that GSHn is associated with decreased oxidative signaling and stress responses and that whole cell redox homeostasis is restored as the cell cycle progresses by enhanced GSH synthesis and accumulation in the cytoplasm. Arabidopsis cells were harvested at points during cell proliferation where GSH was localized either in the nucleus (GSHn) or where GSH was distributed throughout the cytoplasm (GSHc) for RNA extraction and hybridization on Affymetrix microarrays. We selected three stages where the GSH was into the nucleus and three stages where the GSH was distributed throughout the cells.
2010-02-27 | E-GEOD-20454 | biostudies-arrayexpress
Project description:Glutathione (GSH) is a tripeptide involved in controlling heavy metal movement in plants. Our previous study demonstrated that GSH, applied to plant roots site-specifically, inhibited Cd translocation from roots to shoots in oilseed rape plants (Brassica napus) cultured hydroponically. One of the factors of this inhibitory effect was due to activation of Cd efflux from root cells. To investigate the molecular mechanism triggered by root applied GSH in more detail, the Cd movement was monitored non-invasively using a positron-emitting tracer imaging system (PETIS). The Cd absorption and efflux process in roots were visualized successfully. The effects of GSH on Cd efflux from root cells were estimated by analyzing obtained imaging data. Another image analysis suggested that Cd return was activated by GSH, applied to roots, at the shoot base. Cutting the shoot base of oilseed rape plants significantly inhibited Cd efflux from root cells. These experimental results demonstrated the shoot base is playing important roles in distributing Cd in the plant bodies. Furthermore, DNA microarray analysis revealed that over 300 genes in the roots of oilseed rape plants responded to root applied GSH. Among them, transporter proteins, related to heavy metal movement in plants, and proteins related to changing the structure of cell walls were involved.
2021-03-11 | GSE144768 | GEO
Project description:Glutathione is a tripeptide involved in diverse aspects of plant metabolism. We investigated how the reduced form of glutathione, GSH, applied site-specifically to plants, affects zinc (Zn) distribution and behavior in oilseed rape plants (Brassica napus) cultured hydroponically. Foliar-applied GSH significantly increased the Zn content in shoots and the root-to-shoot Zn translocation ratio; furthermore, this treatment raised the Zn concentration in the cytosol of root cells and substantially enhanced Zn xylem loading. Notably, microarray analysis revealed that the gene encoding pectin methylesterase was upregulated in roots following foliar GSH treatment. We conclude that certain physiological signals triggered in response to foliar-applied GSH were transported via sieve tubes and functioned in root cells, which, in turn, increased Zn availability in roots by releasing Zn from their cell wall. Consequently, root-to-shoot translocation of Zn was activated and Zn accumulation in the shoot was markedly increased.
2019-05-29 | GSE109282 | GEO
Project description:The essential thiol antioxidant, glutathione (GSH) is recruited into the nucleus of mammalian cells early in cell proliferation, suggesting a key role of the nuclear thiol pool in cell cycle regulation. However, the functions of nuclear GSH (GSHn) and its integration with the cytoplasmic GSH (GSHc) pools in whole cell redox homeostasis and signaling are unknown. Here we show that GSH is recruited into the nucleus early in cell proliferation in Arabidopsis thaliana, confirming the requirement for localization of GSH in the nucleus as a universal feature of cell cycle regulation. GSH accumulation in the nucleus was triggered by treatments that synchronize cells at G1/S as identified by flow cytometry and marker transcripts. Significant decreases in transcripts associated with oxidative signaling and stress tolerance occurred when GSH was localized in the nucleus. Increases in GSH1 and GSH2 transcripts accompanied the large increase in total cellular GSH observed during cell proliferation, but only GSH2 was differentially expressed in cells with high GSHn relative to those with an even intracellular distribution of GSH. Of the 7 Bcl-2 associated (BAG) genes in A. thaliana, only the nuclear-localized BAG 6 was differentially expressed in cells with high GSHn compared to GSHc. We conclude that GSHn is associated with decreased oxidative signaling and stress responses and that whole cell redox homeostasis is restored as the cell cycle progresses by enhanced GSH synthesis and accumulation in the cytoplasm.
2010-02-22 | GSE20454 | GEO
Project description:Gene expression profiling reveals multiple tissue-specific functionality of GSH. We evaluated the effects of GSH against hydrogen peroxide (HP) on HepG2 cells. We performed an untargeted whole-genome transcriptome analysis to explore functionality of GSH.
2024-03-20 | GSE242415 | GEO