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Circulating MicroRNAs and Treatment Response in Childhood Asthma

ABSTRACT: Total RNA was isolated from serum samples by the Qiagen miRNeasy Serum/Plasma extraction kit and QIAcube automation. All samples were quantified using the Nanodrop spectrophotometer prior to plating. Small RNA-seq libraries were prepared using the Norgen Biotek Small RNA Library Prep Kit and then sequenced on the Illumina NextSeq 500 platform at 51bp single end reads. ExceRpt was employed to assess the read quality and annotate miRNAs. The read count was log transformed and normalized by quantile normalization.

ORGANISM(S): Homo sapiens

PROVIDER: GSE134897 | GEO | 2020/07/08

REPOSITORIES: GEO

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