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Global translation during early development depends on the essential transcription factor PRDM10

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE135022 | GEO | 2020/05/06

REPOSITORIES: GEO

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Global translation during early development depends on the essential transcription factor PRDM10 [RNA-Seq_2]

Project description:Members of the PR/SET domain-containing (PRDM) family of zinc finger transcriptional regulators play diverse roles in embryonic development and lineage differentiation. PRDM10 is a yet uncharacterized family member, with unknown functions in vivo. Here we report an essential requirement for PRDM10 in the pre-implantation embryo and embryonic stem cells (mESCs), where loss of PRDM10 results in severe cell growth inhibition. Genomic and biochemical analyses reveal that PRDM10 functions as a sequence-specific transcription factor which modulates gene expression by binding to target gene promoters. We identify Eif3b, which encodes a core component of the eukaryotic translation initiation factor 3 (eIF3) complex, as a key downstream target and demonstrate that growth inhibition in PRDM10-deficient mESCs is largely mediated through EIF3B-dependent effects on global translation. This work elucidates the molecular function of PRDM10 in maintaining global translation and establishes its essential role in early embryonic development and mESC homeostasis, offering novel insights into the functional repertoire of PRDMs as well as the transcriptional mechanisms regulating global translation.

2020-05-06 | GSE142600 | GEO

Global translation during early development depends on the essential transcription factor PRDM10 [RNA-seq]

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Global translation during early development depends on the essential transcription factor PRDM10 [ChIP-seq]

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Global translation during early development depends on the essential transcription factor PRDM10

Project description:Global translation during early development depends on the essential transcription factor PRDM10

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Global translation during early development depends on the essential transcription factor PRDM10 [RNA-seq]

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Global translation during early development depends on the essential transcription factor PRDM10 [RNA-Seq_2]

Project description:Global translation during early development depends on the essential transcription factor PRDM10 [RNA-Seq_2]

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Global translation during early development depends on the essential transcription factor PRDM10 [ChIP-seq]

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An essential role for heat shock transcription factor binding protein 1 (HSBP1) during early embryonic development.

Project description:Heat shock factor binding protein 1 (HSBP1) is a 76 amino acid polypeptide that contains two arrays of hydrophobic heptad repeats and was originally identified through its interaction with the oligomerization domain of heat shock factor 1 (Hsf1), suppressing Hsf1's transcriptional activity following stress. To examine the function of HSBP1 in vivo, we generated mice with targeted disruption of the hsbp1 gene and examined zebrafish embryos treated with HSBP1-specific morpholino oligonucleotides. Our results show that hsbp1 is critical for preimplantation embryonic development. Embryonic stem (ES) cells deficient in hsbp1 survive and proliferate normally into the neural lineage in vitro; however, lack of hsbp1 in embryoid bodies (EBs) leads to disorganization of the germ layers and a reduction in the endoderm-specific markers (such as ?-fetoprotein). We further show that hsbp1-deficient mouse EBs and knockdown of HSBP1 in zebrafish leads to an increase in the expression of the neural crest inducers Snail2, Tfap2? and Foxd3, suggesting a potential role for HSBP1 in the Wnt pathway. The hsbp1-deficient ES cells, EBs and zebrafish embryos with reduced HSBP1 levels exhibit elevated levels of Hsf1 activity and expression of heat shock proteins (Hsps). We conclude that HSBP1 plays an essential role during early mouse and zebrafish embryonic development.

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Essential control of early B-cell development by Mef2 transcription factors

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-12-14 | E-GEOD-73563 | biostudies-arrayexpress