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Global translation during early development depends on the essential transcription factor PRDM10 [RNA-Seq_2]

ABSTRACT: Global translation during early development depends on the essential transcription factor PRDM10 [RNA-Seq_2]

PROVIDER: PRJNA597726 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10767300.fastq.gz	Fastqsanger.gz
	SRR10767301.fastq.gz	Fastqsanger.gz
	SRR10767302.fastq.gz	Fastqsanger.gz
	SRR10767303.fastq.gz	Fastqsanger.gz
	SRR10767304.fastq.gz	Fastqsanger.gz

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Global translation during early development depends on the essential transcription factor PRDM10 [RNA-Seq_2]

Project description:Members of the PR/SET domain-containing (PRDM) family of zinc finger transcriptional regulators play diverse roles in embryonic development and lineage differentiation. PRDM10 is a yet uncharacterized family member, with unknown functions in vivo. Here we report an essential requirement for PRDM10 in the pre-implantation embryo and embryonic stem cells (mESCs), where loss of PRDM10 results in severe cell growth inhibition. Genomic and biochemical analyses reveal that PRDM10 functions as a sequence-specific transcription factor which modulates gene expression by binding to target gene promoters. We identify Eif3b, which encodes a core component of the eukaryotic translation initiation factor 3 (eIF3) complex, as a key downstream target and demonstrate that growth inhibition in PRDM10-deficient mESCs is largely mediated through EIF3B-dependent effects on global translation. This work elucidates the molecular function of PRDM10 in maintaining global translation and establishes its essential role in early embryonic development and mESC homeostasis, offering novel insights into the functional repertoire of PRDMs as well as the transcriptional mechanisms regulating global translation.

2020-05-06 | GSE142600 | GEO

Global translation during early development depends on the essential transcription factor PRDM10 [RNA-seq]

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Global translation during early development depends on the essential transcription factor PRDM10 [ChIP-seq]

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Global translation during early development depends on the essential transcription factor PRDM10

Project description:Global translation during early development depends on the essential transcription factor PRDM10

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Global translation during early development depends on the essential transcription factor PRDM10 [ChIP-seq]

Project description:Global translation during early development depends on the essential transcription factor PRDM10 [ChIP-seq]

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Global translation during early development depends on the essential transcription factor PRDM10 [RNA-seq]

Project description:Global translation during early development depends on the essential transcription factor PRDM10 [RNA-seq]

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Global translation during early development depends on the essential transcription factor PRDM10.

Project description:Members of the PR/SET domain-containing (PRDM) family of zinc finger transcriptional regulators play diverse developmental roles. PRDM10 is a yet uncharacterized family member, and its function in vivo is unknown. Here, we report an essential requirement for PRDM10 in pre-implantation embryos and embryonic stem cells (mESCs), where loss of PRDM10 results in severe cell growth inhibition. Detailed genomic and biochemical analyses reveal that PRDM10 functions as a sequence-specific transcription factor. We identify Eif3b, which encodes a core component of the eukaryotic translation initiation factor 3 (eIF3) complex, as a key downstream target, and demonstrate that growth inhibition in PRDM10-deficient mESCs is in part mediated through EIF3B-dependent effects on global translation. Our work elucidates the molecular function of PRDM10 in maintaining global translation, establishes its essential role in early embryonic development and mESC homeostasis, and offers insights into the functional repertoire of PRDMs as well as the transcriptional mechanisms regulating translation.

| S-EPMC7368010 | biostudies-literature

Transcriptome analysis of Prdm10 maternal knock out mouse oocytes

Project description:PRDM proteins are metazoan specific transcriptional regulators that play diverse roles in mammalian development and disease. Several members such as PRDM1, PRDM14 and PRDM9, have been implicated in germ cell formation and homeostasis and are essential to fertility-related processes. Others, such as PRDM14, PRDM15 and PRDM10 play a role in early embryogenesis and embryonic stem cell maintenance. Here, we reveal an additional maternal requirement for PRDM10 . Absence of maternal Prdm10 results in catastrophic failure of oocyte-to-embryo transition and complete arrest at the 2-cell stage. We describe multiple defects in oocytes, zygotes and 2-cell stage embryos relating to the failure to accumulate PRDM10 target gene transcripts in the egg. Transcriptomic analysis and integration of genome-wide chromatin-binding data reveals novel, essential PRDM10 targets, including the cytoskeletal protein SEPTIN11. We demonstrate that the failure to express maternal Septin11, in the absence of maternal PRDM10, disrupts Septin-complex assembly at the polar body extrusion site in MII oocytes. Our study sheds light into the essentiality of maternal PRDM10, the requirement of the maternal Septin-complex and the likely evolutionary conservation of this regulatory axis in human female germ cells.

2025-02-07 | GSE283055 | GEO

Global translation during early development depends on the essential transcription factor PRDM10

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-05-06 | GSE135022 | GEO

Transcriptome analysis of Prdm10 maternal knock out mouse oocytes

Project description:Transcriptome analysis of Prdm10 maternal knock out mouse oocytes

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