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Global landscape mapping of chromatin accessibility in DNA damage-induced senescent human stromal cells

ABSTRACT: We report the application of an improved assay for transposase-accessible chromatin with high-throughput sequencing in profiling changes of chromatin openning state in senescent human stromal cells. By obtaining over four billion bases of sequence from Tn5 transposase-processed chromosome DNAs, we generated genome-wide chromatin-state maps of human primary stromal cells (with a starting cell number of 50,000). This study provides a framework for the application of ATAC-Seq technique towards spatiotemporal chromatin configuration of human stromal cells upon DNA damage-induced senescence.

ORGANISM(S): Homo sapiens

PROVIDER: GSE135481 | GEO | 2021/03/23

REPOSITORIES: GEO

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