Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression profiles of long noncoding RNAs and mRNAs in A549 cells infected by Influenza virus

ABSTRACT: Long noncoding RNAs (lncRNAs) participate in regulating many biological processes. However, their roles in influenza A virus (IAV) pathogenicity are largely unknown. Here, we analyzed the expression profile of lncRNAs and mRNAs in the H3N2-infected cells and H7N9-infected cells by high-throughput sequencing

ORGANISM(S): Homo sapiens

PROVIDER: GSE135482 | GEO | 2020/05/13

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptomic characterization of the novel avian-origin influenza A (H7N9) virus: specific and intermediate host-response between avian (H5N1 and H7N7) and human (H3N2) viruses.

Project description:A novel avian-origin H7N9 influenza A virus (IAV) emerged in China in early 2013 causing mild to lethal human respiratory infections. H7N9 originated from multiple reassortment events between avian viruses and carries genetic markers of human adaptation. Determining whether H7N9 induces a host-response closer to human or avian IAV is important to better characterize this emerging virus. Here we compared the human lung epithelial cell response to infection with A/Anhui/01/13 (H7N9) or highly pathogenic avian-origin H5N1, H7N7, or human seasonal H3N2 IAV. Here, polarized confluent monolayers of Calu-3 cells were infected apically with the avian-origin IAVs A/Anhui/01/2013 (H7N9) [Anhui01], A/Netherland/219/2003 (H7N7) [NL219], A/Vietnam/1203/2004 (H5N1) [VN1203], or a human seasonal virus A/Panama/2007/1999 (H3N2) [Pan99] at an MOI of 1. Time-matched mocks were also included using the same cell stock as the rest of the samples. Culture medium (same as what the virus stock is in) was used for the mock infections. Quadruplicate wells were infected for each virus/timepoint. Measured timepoints were 3, 7, 12 and 24 hours post-inoculation and the RNA was used for transcriptional analysis via microarray.

2014-01-16 | E-GEOD-49840 | biostudies-arrayexpress

Transcriptomic characterization of the novel avian-origin influenza A (H7N9) virus: specific and intermediate host-response between avian (H5N1 and H7N7) and human (H3N2) viruses.

2014-01-16 | GSE49840 | GEO

Expression profiles of circular RNA in A549 cells infected by Influenza virus

Project description:Circular RNAs (circRNAs) participate in regulating many biological processes. However, their roles in influenza A virus (IAV) pathogenicity are largely unknown. Here, we analyzed the expression profile of circRNAs in the H1N1-infected cells by high-throughput sequencing

2023-08-07 | GSE181727 | GEO

Expression profiles of long noncoding RNAs and mRNAs in ST cells infected by PDCoV

Project description:Long noncoding RNAs (lncRNAs) participate in regulating many biological processes. However, their roles in PDCoV pathogenicity are largely unknown. Here, we analyzed the expression profile of lncRNAs and mRNAs in the PDCoV-infected cells by high-throughput sequencing

2022-08-28 | GSE136705 | GEO

RNA-seq data of lungs from mouse infected with different H5N1 Avian Influenza Viruses

Project description:Long non-coding RNAs (lncRNAs) play multiple key regulatory roles in various biological processes. However, their function in influenza A virus (IAV) pathogenicity remains largely unexplored. Here, using next generation sequencing, we systemically compared the whole-transcriptome response of the mouse lung infected either with high-pathogenic (A/Chicken/Jiangsu/k0402/2010, CK10) or with nonpathogenic (A/Goose/Jiangsu/k0403/2010, GS10) H5N1 strains. A total of 370 significantly differentially expressed (DE) lncRNAs from three replicates were identified associated with the high virulence of CK10, whereas the corresponding number for GS10 was 229.

2018-09-06 | GSE100522 | GEO

Transcription profiling by array to investigate the response of mice colon samples when infected by a H3N2 influenza virus.

Project description:To study the impact of influenza infection on gene expression changes in the colon, mice were intra-nasally infected with 30 plaque forming units of the mouse-adapted H3N2 IAV strain Scotland/20/1974. PBS-treated mice served as controls. The colons were collected 7 days post-infection.

2019-12-01 | E-MTAB-6707 | biostudies-arrayexpress

Expression profiles of long noncoding RNAs and mRNAs in primary porcine macrophages (PAMs) Infected with African swine fever virus

Project description:Long noncoding RNAs (lncRNAs) participate in regulating many biological processes. However, their roles in African swine fever virus(ASFV) pathogenicity are largely unknown. Here, we analyzed the expression profile of lncRNAs and mRNAs in the ASFV-infected or uninfected PAMs by high-throughput sequencing

2024-07-27 | GSE180939 | GEO

Influenza A virus

Project description:Receptor binding of antigenically drifted H3N2 IAV

| PRJNA561570 | ENA

Analysis of Expression Profiles of long noncoding RNAs in A549 cells infected by H1N1 [lncRNA-seq]

Project description:Although accumulating evidence has shown that long non-coding RNAs (lncRNAs) are involved in multiple biological processes, considerably less is known regarding their functions in influenza A virus (IAV) replication. Here, lncRNA expression profiles were determined by RNA sequencing in three pairs of influenza virus A/Puerto Rico/8/34 (H1N1)-infected or uninfected A549 cells.

2023-03-31 | GSE208546 | GEO

Identification of influenza virus-encoded vcircRNAs in human A549 cells by RNA-seq

Project description:The discovery of noncoding RNAs is reshaping paradigms in molecular biology and virology. Circular RNAs (circRNAs), a class of novel noncoding RNAs, their circular conformation endows these transcripts with critical physiological and pathological roles. Here, we employed circRNAs sequencing technologies, following RNase R treatment on influenza virus-infected human pulmonary epithelial cells to identify and characterize influenza virus-derived circRNAs. Back spicing junctions mapping analysis identified lots of circRNAs from 7 viral gene segment apart from M gene and subsequent experiments confirmed the presence of these IAV-encoded circRNAs in infected cells and mice lung. We found that polymerase genes could encode numerous viral circRNA (vcircRNAs) within infected cells, such as NP and PB1. By vcircRNA overexpression and knockdown with small interfering RNA, we found that these vcircRNAs is required for efficient viral replication. Notably, IAV-derived circRNAs biogenesis was formed with canonical GU/AG splicing mechanism in infected A549 cells. Splicing site mutant viruses displayed a significant decrease in the viral replication in vitro and pathogenicity in mice. Overall, our findings will not only uncover the novel RNA transcripts in IAVs infection cycle, but also provide a new perspective to investigate the crosstalk between host and IAVs from vcircRNAs.

2024-12-05 | GSE283221 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data